Cited 17 times in
The effect of PLGA sphere diameter on rabbit mesenchymal stem cells in adipose tissue engineering
DC Field | Value | Language |
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dc.contributor.author | 박시내 | - |
dc.contributor.author | 서활 | - |
dc.contributor.author | 최유석 | - |
dc.date.accessioned | 2015-05-19T16:52:29Z | - |
dc.date.available | 2015-05-19T16:52:29Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0957-4530 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107102 | - |
dc.description.abstract | To investigate the effect of injectable PLGA sphere's diameter on adipose tissue engineering, rabbit mesenchymal stem cells were attached to various diameters of injectable PLGA spheres (<75; 75-100; 100-150; 150-200; and 200-250 microm). These five groups were cultured in adipogenic media for 2 weeks in vitro and injected into necks of nude mice. Prior to in vivo study, cell proliferation and adipogenic differentiation were determined by hexosaminidase assay and Oil red O staining after 2 weeks. Group C (100-150 microm) showed the highest adipogenic differentiation and the proliferation capacity of Group B (75-100 microm) was significantly higher than that of any other group. We harvested newly formed tissues from necks of nude mice after 1 and 4 weeks. Although PLGA spheres have not been degraded and there was no significant histological difference among various sizes of spheres after 1 week, well-organized fat pads (PLGA spheres were completely degraded) could be observed, and the histology of the 100-150 microm groups resembled that of native tissue after 4 weeks. Based on these experiments, we could conclude that the optimal size of PLGA spheres for adipogenesis was 100-150 microm | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2165~2171 | - |
dc.relation.isPartOf | JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/cytology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Azo Compounds/pharmacology | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Green Fluorescent Proteins/metabolism | - |
dc.subject.MESH | Hexosaminidases/chemistry | - |
dc.subject.MESH | Lactic Acid/chemistry* | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Microscopy, Electron, Scanning | - |
dc.subject.MESH | Microspheres | - |
dc.subject.MESH | Polyglycolic Acid/chemistry* | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Tissue Engineering/methods | - |
dc.title | The effect of PLGA sphere diameter on rabbit mesenchymal stem cells in adipose tissue engineering | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학) | - |
dc.contributor.googleauthor | Yu Suk Choi | - |
dc.contributor.googleauthor | Si-Nae Park | - |
dc.contributor.googleauthor | Hwal Suh | - |
dc.identifier.doi | 10.1007/s10856-007-3320-2 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01552 | - |
dc.contributor.localId | A01924 | - |
dc.contributor.localId | A04134 | - |
dc.relation.journalcode | J01576 | - |
dc.identifier.eissn | 1573-4838 | - |
dc.identifier.pmid | 18040757 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10856-007-3320-2 | - |
dc.subject.keyword | Adipose Tissue | - |
dc.subject.keyword | Green Fluorescence Protein | - |
dc.subject.keyword | Nude Mouse | - |
dc.subject.keyword | Adipogenic Differentiation | - |
dc.subject.keyword | PLLA Scaffold | - |
dc.contributor.alternativeName | Park, Si Nae | - |
dc.contributor.alternativeName | Suh, Hwal | - |
dc.contributor.alternativeName | Choi, Yu Suk | - |
dc.contributor.affiliatedAuthor | Park, Si Nae | - |
dc.contributor.affiliatedAuthor | Suh, Hwal | - |
dc.contributor.affiliatedAuthor | Choi, Yu Suk | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 2165 | - |
dc.citation.endPage | 2171 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, Vol.19(5) : 2165-2171, 2008 | - |
dc.identifier.rimsid | 49535 | - |
dc.type.rims | ART | - |
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