1 339

Cited 0 times in

Tumor-targeted radionuclide imaging and therapy based on human sodium iodide symporter gene driven by a modified telomerase reverse transcriptase promoter

DC Field Value Language
dc.contributor.author윤채옥-
dc.date.accessioned2015-05-19T16:45:33Z-
dc.date.available2015-05-19T16:45:33Z-
dc.date.issued2008-
dc.identifier.issn1043-0342-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/106891-
dc.description.abstractHuman telomerase reverse transcriptase (hTERT) is highly active in most cancer cells and, thus, could be used for tumor targeting. The human sodium iodide symporter (hNIS) gene is being actively researched as a potential radioactive iodine (radioiodine) gene therapy. In this study, we investigated the possibilities of using the hNIS gene driven by the hTERT promoter for molecular imaging and radioiodine gene therapy. Stable cell lines of hTERT-positive cells (Hep3B hepatoma) expressing hNIS, under the control of the 5mmTERT promoter, were generated using a retroviral system. Radioiodine uptake and efflux tests were performed, and a clonogenic assay was used to evaluate the in vitro cytotoxicity of 131I. Finally, scintigraphic, biodistribution, and radioiodine therapy studies were performed in vivo. Radioiodine uptake by 5mmTERT-NIS-transfected Hep3B cells was 22 times higher than by nontransfected Hep3B cells, and 5 times that of 5mmTERT-NIS-transfected U2-OS cells (p < 0.05). Clonogenic assays demonstrated that the survival rate of Hep3B-5mmTERT-NIS cells after 131I incubation was significantly lower than that of Hep3B cells (p < 0.001), and radioiodine accumulations in Hep3B-5mmTERT-NIS tumors were significantly higher than in wild-type tumors. In addition, technetium- 99m scintigraphy clearly visualized Hep3B-5mmTERT-NIS tumors. Moreover, after being treated with 111 MBq of 131I-labeled Hep3B-5mmTERT-NIS, tumor growth was retarded, whereas Hep3B tumor growth progressed. hTERT-positive tumors were successfully targeted by the NIS gene under the control of the 5mmTERT promoter. The described system could be useful for targeted molecular imaging and as a radioiodine gene therapy for cancer.-
dc.description.statementOfResponsibilityopen-
dc.format.extent951~957-
dc.relation.isPartOfHUMAN GENE THERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHGenetic Therapy/methods*-
dc.subject.MESHGenetic Vectors-
dc.subject.MESHHumans-
dc.subject.MESHIodine Radioisotopes/therapeutic use*-
dc.subject.MESHLiver Neoplasms, Experimental/diagnostic imaging-
dc.subject.MESHLiver Neoplasms, Experimental/radiotherapy-
dc.subject.MESHLiver Neoplasms, Experimental/therapy-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHNeoplasm Transplantation-
dc.subject.MESHOsteosarcoma/diagnostic imaging-
dc.subject.MESHOsteosarcoma/radiotherapy-
dc.subject.MESHOsteosarcoma/therapy-
dc.subject.MESHPlasmids/genetics-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHRadionuclide Imaging-
dc.subject.MESHRetroviridae/genetics-
dc.subject.MESHSymporters/genetics*-
dc.subject.MESHTelomerase/genetics*-
dc.subject.MESHTransfection-
dc.subject.MESHTransplantation, Heterologous-
dc.titleTumor-targeted radionuclide imaging and therapy based on human sodium iodide symporter gene driven by a modified telomerase reverse transcriptase promoter-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentInstitute for Cancer Research (암연구소)-
dc.contributor.googleauthorSeung Hoo Kim-
dc.contributor.googleauthorHye Kyung Chung-
dc.contributor.googleauthorJoo Hyun Kang-
dc.contributor.googleauthorKwang Il Kim-
dc.contributor.googleauthorYong Hyun Jeon-
dc.contributor.googleauthorYong Nan Jin-
dc.contributor.googleauthorChae Ok Yun-
dc.contributor.googleauthorJune-Key Chung-
dc.identifier.doi10.1089/hgt.2008.030-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02614-
dc.relation.journalcodeJ01006-
dc.identifier.eissn1557-7422-
dc.identifier.pmid18810799-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/hum.2008.030-
dc.subject.keywordAnimals-
dc.subject.keywordBase Sequence-
dc.subject.keywordCell Line, Tumor-
dc.subject.keywordGenetic Therapy/methods*-
dc.subject.keywordGenetic Vectors-
dc.subject.keywordHumans-
dc.subject.keywordIodine Radioisotopes/therapeutic use*-
dc.subject.keywordLiver Neoplasms, Experimental/diagnostic imaging-
dc.subject.keywordLiver Neoplasms, Experimental/radiotherapy-
dc.subject.keywordLiver Neoplasms, Experimental/therapy-
dc.subject.keywordMice-
dc.subject.keywordMice, Nude-
dc.subject.keywordNeoplasm Transplantation-
dc.subject.keywordOsteosarcoma/diagnostic imaging-
dc.subject.keywordOsteosarcoma/radiotherapy-
dc.subject.keywordOsteosarcoma/therapy-
dc.subject.keywordPlasmids/genetics-
dc.subject.keywordPromoter Regions, Genetic-
dc.subject.keywordRadionuclide Imaging-
dc.subject.keywordRetroviridae/genetics-
dc.subject.keywordSymporters/genetics*-
dc.subject.keywordTelomerase/genetics*-
dc.subject.keywordTransfection-
dc.subject.keywordTransplantation, Heterologous-
dc.contributor.alternativeNameYun, Chae Ok-
dc.contributor.affiliatedAuthorYun, Chae Ok-
dc.rights.accessRightsnot free-
dc.citation.volume19-
dc.citation.number9-
dc.citation.startPage951-
dc.citation.endPage957-
dc.identifier.bibliographicCitationHUMAN GENE THERAPY, Vol.19(9) : 951-957, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.