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High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes.
DC Field | Value | Language |
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dc.contributor.author | 김은숙 | - |
dc.contributor.author | 신전수 | - |
dc.contributor.author | 윤주호 | - |
dc.date.accessioned | 2015-05-19T16:38:39Z | - |
dc.date.available | 2015-05-19T16:38:39Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106680 | - |
dc.description.abstract | LPS-binding protein (LBP) is a central mediator that transfers LPS to CD14 to initiate TLR4-mediated proinflammatory response. However, a possibility of another LPS transfer molecule has been suggested because LBP-deficient mice showed almost normal inflammatory response after LPS injection. In this study, we describe the novel finding that high mobility group box 1 protein (HMGB1) recently identified as a mediator of sepsis has a function of LPS transfer for a proinflammatory response. We used ELISA and surface plasmon resonance to show that HMGB1 binds LPS in a concentration-dependent manner and that the binding is stronger to lipid A moiety than to the polysaccharide moiety of LPS. This binding was inhibited by LBP and polymyxin B. Using native PAGE and fluorescence-based LPS transfer analyses, we show that HMGB1 can catalytically disaggregate and transfer LPS to both soluble CD14 protein and to human PBMCs in a dose-dependent manner. However, this effect was dramatically reduced to the baseline level when HMGB1 was heat inactivated. Furthermore, a mixture of HMGB1 and LPS treatment results in a higher increase in TNF-alpha production in human PBMCs and peripheral blood monocytes than LPS or HMGB1 treatment alone or their summation. Thus, we propose that HMGB1 plays an important role in Gram-negative sepsis by catalyzing movement of LPS monomers from LPS aggregates to CD14 to initiate a TLR4-mediated proinflammatory response | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 5067~5074 | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | CHO Cells | - |
dc.subject.MESH | Catalysis | - |
dc.subject.MESH | Cricetinae | - |
dc.subject.MESH | Cricetulus | - |
dc.subject.MESH | HMGB1 Protein/genetics | - |
dc.subject.MESH | HMGB1 Protein/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Leukocytes/drug effects | - |
dc.subject.MESH | Leukocytes/metabolism | - |
dc.subject.MESH | Lipopolysaccharide Receptors/metabolism* | - |
dc.subject.MESH | Lipopolysaccharides/pharmacology* | - |
dc.subject.MESH | Micelles | - |
dc.subject.MESH | Monocytes/drug effects* | - |
dc.subject.MESH | Monocytes/metabolism* | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Solubility | - |
dc.subject.MESH | Surface Plasmon Resonance | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/biosynthesis* | - |
dc.title | High mobility group box 1 protein binding to lipopolysaccharide facilitates transfer of lipopolysaccharide to CD14 and enhances lipopolysaccharide-mediated TNF-alpha production in human monocytes. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Ju Ho Youn | - |
dc.contributor.googleauthor | Young Joo Oh | - |
dc.contributor.googleauthor | Eun Sook Kim | - |
dc.contributor.googleauthor | Ji Eun Choi | - |
dc.contributor.googleauthor | Jeon-Soo Shin | - |
dc.identifier.doi | 18354232 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02144 | - |
dc.contributor.localId | A02605 | - |
dc.contributor.localId | A00808 | - |
dc.relation.journalcode | J01450 | - |
dc.identifier.eissn | 1550-6606 | - |
dc.identifier.pmid | 18354232 | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | CHO Cells | - |
dc.subject.keyword | Catalysis | - |
dc.subject.keyword | Cricetinae | - |
dc.subject.keyword | Cricetulus | - |
dc.subject.keyword | HMGB1 Protein/genetics | - |
dc.subject.keyword | HMGB1 Protein/metabolism* | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Leukocytes/drug effects | - |
dc.subject.keyword | Leukocytes/metabolism | - |
dc.subject.keyword | Lipopolysaccharide Receptors/metabolism* | - |
dc.subject.keyword | Lipopolysaccharides/pharmacology* | - |
dc.subject.keyword | Micelles | - |
dc.subject.keyword | Monocytes/drug effects* | - |
dc.subject.keyword | Monocytes/metabolism* | - |
dc.subject.keyword | Protein Binding | - |
dc.subject.keyword | Solubility | - |
dc.subject.keyword | Surface Plasmon Resonance | - |
dc.subject.keyword | Tumor Necrosis Factor-alpha/biosynthesis* | - |
dc.contributor.alternativeName | Kim, Eun Sook | - |
dc.contributor.alternativeName | Shin, Jeon Soo | - |
dc.contributor.alternativeName | Youn, Ju Ho | - |
dc.contributor.affiliatedAuthor | Shin, Jeon Soo | - |
dc.contributor.affiliatedAuthor | Youn, Ju Ho | - |
dc.contributor.affiliatedAuthor | Kim, Eun Sook | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 180 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 5067 | - |
dc.citation.endPage | 5074 | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, Vol.180(7) : 5067-5074, 2008 | - |
dc.identifier.rimsid | 46654 | - |
dc.type.rims | ART | - |
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