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Preservation of platelets by adding epigallocatechin-3-o-gallate to platelet concentrates

Authors
 Kazuaki Matsumura  ;  Hiroshi Takayama  ;  Jung Yoon Bae  ;  Mitsuru Kurihara  ;  Sadami Tsutsumi  ;  Suong-Hyu Hyon 
Citation
 CELL TRANSPLANTATION, Vol.18(5-6) : 521-528, 2009 
Journal Title
 CELL TRANSPLANTATION 
ISSN
 0963-6897 
Issue Date
2009
MeSH
Antioxidants/pharmacology* ; Apoptosis ; Blood Platelets/drug effects* ; Blood Platelets/physiology ; Blood Preservation/methods* ; Caspase 3/metabolism ; Catechin/analogs & derivatives* ; Catechin/pharmacology ; Chemokine CCL5/secretion ; Gelsolin/metabolism ; Humans ; L-Lactate Dehydrogenase/metabolism ; P-Selectin/metabolism ; Thromboplastin/metabolism
Abstract
The effect of epigallocatechin-3-O-gallate (EGCG), a major component of green tea, on platelet preservation was evaluated. Single donor platelets (N = 10) were collected and preserved by the standard method. EGCG was added to the platelet concentrates before preservation and then the functional and biochemical parameters were monitored throughout the storage period. After 6 days of preservation, the aggregability of the platelets was significantly maintained by addition of 50 and 100 microg/ml of EGCG. Platelet prothrombinase activity was also significantly retained by the addition of EGCG. The accumulation of P-selectin and RANTES in the plasma preserved with EGCG was less than those preserved without EGCG, which indicated that EGCG might inhibit platelet activation. Furthermore, EGCG reduced the increase of LDH in plasma during preservation and inhibited the activation of caspase-3 and cleavage of gelsolin, thereby showing that EGCG could inhibit the apoptosis of platelets. These results suggest that EGCG may play an effective role in preserving platelets by inhibiting the activation and apoptosis of platelets.
Full Text
http://www.ingentaconnect.com/content/cog/ct/2009/00000018/F0020005/art00006
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Bae, Jung Yoon(배정윤) ORCID logo https://orcid.org/0000-0001-8342-6987
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/106068
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