Cited 13 times in
Production of and applications for a polyclonal IgY diagnostic reagent specific for Mycobacterium avium subsp. Paratuberculosis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신성재 | - |
dc.date.accessioned | 2015-04-24T17:46:29Z | - |
dc.date.available | 2015-04-24T17:46:29Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106044 | - |
dc.description.abstract | Antibodies specific to the cell surface antigens of Mycobacterium avium subsp. paratuberculosis (MAP) have multiple useful applications, e.g. organism detection, immunoconcentration, and cell visualization. The aim of this study was to produce and compare polyclonal antibodies for such research and diagnostic purposes. Three polyclonal antibodies to MAP were produced using sera from immunized rabbits and chickens plus naturally infected cows. Cross-reactive antibodies in each MAP antibody preparation were removed by absorption with heterologous mycobacterial and non-mycobacterial cells. The specificity of each resulting polyclonal antibody preparation was evaluated by ELISA to multiple bacterial cell wall extract antigens. After absorption, chicken anti-MAP IgY had the highest specificity of the three antibody preparations. FITC-la-beled anti-MAP IgY was used to effectively locate MAP in macrophages 12 h post-infection. Also, immunomagnetic beads coated with anti-MAP IgY enhanced recovery of MAP from bacterial suspensions in comparison with non-antibody coated beads. Anti-MAP IgY provides a novel new reagent with broad diagnostic and research applications requiring specific concentration, detection, and quantification of MAP. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 600~609 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Bacterial/isolation & purification* | - |
dc.subject.MESH | Antibody Specificity | - |
dc.subject.MESH | Cattle | - |
dc.subject.MESH | Chickens | - |
dc.subject.MESH | Fluorescent Antibody Technique, Direct/methods | - |
dc.subject.MESH | Immunoglobulins/isolation & purification* | - |
dc.subject.MESH | Immunomagnetic Separation/methods | - |
dc.subject.MESH | Macrophages/microbiology | - |
dc.subject.MESH | Mycobacterium avium subsp. paratuberculosis/immunology* | - |
dc.subject.MESH | Rabbits | - |
dc.title | Production of and applications for a polyclonal IgY diagnostic reagent specific for Mycobacterium avium subsp. Paratuberculosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.contributor.googleauthor | Seung-Sub Lee | - |
dc.contributor.googleauthor | Elizabeth J. B. Manning | - |
dc.contributor.googleauthor | Michael T. Collins | - |
dc.identifier.doi | 10.1007/s12275-009-0052-7 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02114 | - |
dc.relation.journalcode | J01593 | - |
dc.identifier.eissn | 1976-3794 | - |
dc.identifier.pmid | 19851733 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs12275-009-0052-7 | - |
dc.subject.keyword | M. paratuberculosis | - |
dc.subject.keyword | polyclonal antibodies for diagnostic purposes | - |
dc.subject.keyword | absorption | - |
dc.subject.keyword | anti-MAP IgY | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | Shin, Sung Jae | - |
dc.citation.volume | 47 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 600 | - |
dc.citation.endPage | 609 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, Vol.47(5) : 600-609, 2009 | - |
dc.identifier.rimsid | 56998 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.