Cited 8 times in
Rapid mycobacterial liquid culture-screening method for Mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay
DC Field | Value | Language |
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dc.contributor.author | 신성재 | - |
dc.date.accessioned | 2015-04-24T17:46:25Z | - |
dc.date.available | 2015-04-24T17:46:25Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1556-6811 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/106042 | - |
dc.description.abstract | Sensors in automated liquid culture systems for mycobacteria, such as MGIT, BacT/Alert 3D, and Trek ESP II, flag growth of any type of bacteria; a positive signal does not mean that the target mycobacteria are present. All signal-positive cultures thus require additional and often laborious testing. An immunoassay was developed to screen liquid mycobacterial cultures for evidence of Mycobacterium avium complex (MAC). The method, called the MAC-enzyme-linked immunosorbent assay (ELISA), relies on detection of MAC-specific secreted antigens in liquid culture. Secreted MAC antigens were captured by the MAC-ELISA with polyclonal anti- Mycobacterium avium subsp. paratuberculosis chicken immunoglobulin Y (IgY), detected using rabbit anti-MAC IgG, and then revealed using horseradish peroxidase-conjugated goat anti-rabbit IgG. When the MAC-ELISA was evaluated using pure cultures of known mycobacterial (n = 75) and nonmycobacterial (n = 17) organisms, no false-positive or false-negative MAC-ELISA results were found. By receiver operator characteristic (ROC) analysis of 1,275 previously identified clinical isolates, at the assay optimal cutoff the diagnostic sensitivity and specificity of the MAC-ELISA were 92.6% (95% confidence interval [95% CI], 90.3 to 94.5) and 99.9% (95% CI, 99.2 to 100), respectively, with an area under the ROC curve of 0.992. Prospective evaluation of the MAC-ELISA with an additional 652 clinical samples inoculated into MGIT ParaTB medium and signaling positive per the manufacturer's instructions found that the MAC-ELISA was effective in determining those cultures that actually contained MAC species and warranting the resources required to identify the organism by PCR. Of these 652 MGIT-positive cultures, the MAC-ELISA correctly identified 96.8% (of 219 MAC-ELISA-positive cultures) as truly containing MAC mycobacteria, based on PCR or high-performance liquid chromatography (HPLC) as reference tests. Only 6 of 433 MGIT signal-positive cultures (1.4%) were MAC-ELISA false negative, and only 7 of 219 MGIT signal-negative cultures (3.2%) were false positive. The MAC-ELISA is a low-cost, rapid, sensitive, and specific test for MAC in liquid cultures. It could be used in conjunction with or independent of automated culture reading instrumentation. For maximal accuracy and subspecies-specific identification, use of a confirmatory multiplex MAC PCR is recommended | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 613~620 | - |
dc.relation.isPartOf | Clinical and Vaccine Immunology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antigens, Bacterial/immunology | - |
dc.subject.MESH | Antigens, Bacterial/isolation & purification* | - |
dc.subject.MESH | Diagnostic Errors | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay/methods | - |
dc.subject.MESH | Mass Screening/methods* | - |
dc.subject.MESH | Mycobacterium avium Complex/genetics | - |
dc.subject.MESH | Mycobacterium avium Complex/growth & development | - |
dc.subject.MESH | Mycobacterium avium Complex/isolation & purification* | - |
dc.subject.MESH | Mycobacterium avium-intracellulare Infection/diagnosis | - |
dc.subject.MESH | Mycobacterium avium-intracellulare Infection/microbiology | - |
dc.subject.MESH | Mycobacterium avium-intracellulare Infection/veterinary* | - |
dc.subject.MESH | Paratuberculosis/diagnosis* | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.title | Rapid mycobacterial liquid culture-screening method for Mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.contributor.googleauthor | Kelly Anklam | - |
dc.contributor.googleauthor | Elizabeth J. B. Manning | - |
dc.contributor.googleauthor | Michael T. Collins | - |
dc.identifier.doi | 10.1128/CVI.00461-08 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02114 | - |
dc.relation.journalcode | J00559 | - |
dc.identifier.pmid | 19261776 | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | Shin, Sung Jae | - |
dc.citation.volume | 16 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 613 | - |
dc.citation.endPage | 620 | - |
dc.identifier.bibliographicCitation | Clinical and Vaccine Immunology, Vol.16(5) : 613-620, 2009 | - |
dc.identifier.rimsid | 56996 | - |
dc.type.rims | ART | - |
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