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Prevalence of the Extended-Spectrum beta-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli

Other Titles
 Escherichia coli 의 Extended-Spectrum β-Lactamase 및 qnr 유전자 보유 현황 
 Yongjung Park  ;  Hyun-Kyung Kang  ;  Il Kwon Bae  ;  Juwon Kim  ;  Jae-Seok Kim  ;  Young Uh  ;  Seok Hoon Jeong  ;  Kyungwon Lee 
 Korean Journal of Laboratory Medicine, Vol.29(3) : 218-223, 2009 
Journal Title
 Korean Journal of Laboratory Medicine 
Issue Date
Disk Diffusion Antimicrobial Tests ; Escherichia coli/enzymology* ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Escherichia coli Proteins/classification ; Escherichia coli Proteins/genetics* ; Humans ; Inhibitory Concentration 50 ; Polymerase Chain Reaction ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics ; beta-Lactamases/metabolism*
Escherichia coli ; CTX-M ESBL ; qnrA1 ; qnrB4
BACKGROUND: This study was performed to investigate the prevalence of qnr genes in clinical isolates of Escherichia coli from Korea that produce extended-spectrum beta-lactamases (ESBLs). METHODS: During the period of May to June 2005, we collected clinical isolates of E. coli that were intermediate or resistant to ceftazidime and/or cefotaxime from 11 Korean hospitals. Antimicrobial susceptibility was determined by the disk diffusion and agar dilution methods. ESBL production was confirmed phenotypically by the double-disk synergy test. ESBL and qnr genes were searched for by PCR amplification, and the PCR products were then subjected to direct sequencing. RESULTS: Double-disk synergy tests were positive in 84.3% (118/140) of ceftazidime- and/or cefotaxime-nonsusceptible E. coli isolates. The most prevalent types of ESBL in E. coli isolates were CTX-M-14 (N=41) and CTX-M-15 (N=58). Other ESBLs were also identified, including CTX-M-3 (N=7), CTX-M-9 (N=8), CTX-M-12 (N=1), CTX-M-57 (N=1), SHV-2a (N=2), SHV-12 (N=17) and TEM-52 (N=4). The qnrA1 and qnrB4 genes were identified in 4 and 7 ESBL-producing isolates, respectively. CONCLUSIONS: CTX-M-type enzymes were the most common type of ESBL in E. coli isolates from Korea, and the qnr genes were not uncommon in ESBL-producing E. coli isolates. Dissemination of E. coli containing both ESBL and qnr genes could compromise the future usefulness of the expanded-spectrum antibiotics for the treatment of infections.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Won(김주원)
Bae, Il Kwon(배일권)
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
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