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Effective neuropathic pain relief through sciatic nerve administration of GAD65-expressing Raav2

Authors
 Jaehyung Kim  ;  Sung Jin Kim  ;  Heuiran Lee  ;  Jin Woo Chang 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.388(1) : 73-78, 2009 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2009
MeSH
Adenoviridae* ; Animals ; Disease Models, Animal ; Ganglia, Spinal ; Gene Transfer Techniques ; Genetic Therapy/methods* ; Glutamate Decarboxylase/genetics* ; Male ; Neuralgia/etiology ; Neuralgia/therapy* ; Peripheral Nervous System Diseases/complications ; Peripheral Nervous System Diseases/therapy* ; Rats ; Rats, Sprague-Dawley ; Sciatic Nerve*
Keywords
Neuropathic pain ; Sciatic nerve ; rAAV2 ; GAD65 ; GABA
Abstract
Recently, we demonstrated that the administration of GAD65-expressing rAAV2 to DRG attenuates peripheral neuropathy by inducing GABA release in the spinal cord. However, the direct injection to DRG is invasive and may therefore cause nerve injury and other side effects. To circumvent this surgical intervention, we explored the potential of a much simpler and less invasive route of sciatic nerve administration. Using a neuropathic pain model, we introduced rAAV2-GAD65 through sciatic nerve and examined its therapeutic potency in pain-related behavior tests. Both GFP and GAD65 expression indicated that effective transgene delivery to the DRG can be accomplished via sciatic nerve administration. Equally importantly, the GABA concentration in the spinal cord increased significantly after GAD65 introduction, and pain symptoms were dramatically reduced and persistently controlled. The implication is that the sciatic nerve is a highly promising route for delivering rAAV2 to the DRG, and thus represents a much less invasive, clinically viable gene therapy option.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X0901479X
DOI
10.1016/j.bbrc.2009.07.120
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Chang, Jin Woo(장진우) ORCID logo https://orcid.org/0000-0002-2717-0101
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105825
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