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Identification of the large-conductance background K+ channel in mouse B cells as TREK-2.
DC Field | Value | Language |
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dc.contributor.author | 남주현 | - |
dc.date.accessioned | 2015-04-24T17:38:55Z | - |
dc.date.available | 2015-04-24T17:38:55Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105801 | - |
dc.description.abstract | Mouse B cells and their cell line (WEHI-231) express large-conductance background K(+) channels (LK(bg)) that are activated by arachidonic acids, characteristics similar to TREK-2. However, there is no evidence to identify the molecular nature of LK(bg); some properties of LK(bg) were partly different from the reported results of TREK type channels. In this study, we compared the properties of cloned TREK-2 and LK(bg) in terms of their sensitivities to ATP, phosphatidylinositol 4,5-bisphosphate (PIP(2)), intracellular pH (pH(i)), and membrane stretch. Similar to the previous findings of LK(bg), TREK-2 showed spontaneous activation after membrane excision (i-o patch) and were inhibited by MgATP or by PIP(2). The inhibition by MgATP was prevented by wortmannin, suggesting membrane-delimited regulation of TREKs by phosphoinositide (PI) kinase. The same was observed with the property of LK(bg); the activation of TREK-2 by membrane stretch was suppressed by U73122 (PLC inhibitor). As with the known properties of TREK-2, LK(bg) were activated by acidic pH(i) and inhibited by PKC activator. Finally, we confirmed the expression of TREK-2 in WEHI-231 by using RT-PCR and immunoblot analyses. The amplitude of background K(+) current and the TREK-2 expression in WEHI-231 were commonly decreased by genetic knockdown of TREK-2 using small interfering RNA. The downregulation of TREK-2 attenuated Ca(2+)-influx induced by arachidonic acid in WEHI-231. As a whole, these results strongly indicate that TREK-2 encodes LK(bg) in mouse B cells. We also newly suggest that the low activity of TREK-2 in intact cells is due to the inhibition by intrinsic PIP(2). | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | C188~C197 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 1-Phosphatidylinositol 4-Kinase/metabolism | - |
dc.subject.MESH | Adenosine Triphosphate/metabolism | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Arachidonic Acid/metabolism | - |
dc.subject.MESH | B-Lymphocytes/drug effects | - |
dc.subject.MESH | B-Lymphocytes/enzymology | - |
dc.subject.MESH | B-Lymphocytes/metabolism* | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Shape | - |
dc.subject.MESH | Cloning, Molecular | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen-Ion Concentration | - |
dc.subject.MESH | Mechanotransduction, Cellular | - |
dc.subject.MESH | Membrane Potentials | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Phosphatidylinositol 4,5-Diphosphate/metabolism | - |
dc.subject.MESH | Phosphodiesterase Inhibitors/pharmacology | - |
dc.subject.MESH | Potassium/metabolism* | - |
dc.subject.MESH | Potassium Channels, Tandem Pore Domain/drug effects | - |
dc.subject.MESH | Potassium Channels, Tandem Pore Domain/genetics | - |
dc.subject.MESH | Potassium Channels, Tandem Pore Domain/metabolism* | - |
dc.subject.MESH | Protein Kinase Inhibitors/pharmacology | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Type C Phospholipases/metabolism | - |
dc.title | Identification of the large-conductance background K+ channel in mouse B cells as TREK-2. | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | 생체방어연구센터 | - |
dc.contributor.googleauthor | Haifeng Zheng | - |
dc.contributor.googleauthor | Joo Hyun Nam | - |
dc.contributor.googleauthor | Bo Pang | - |
dc.contributor.googleauthor | Dong Hoon Shin | - |
dc.contributor.googleauthor | Ji Seon Kim | - |
dc.contributor.googleauthor | Yang-Sook Chun | - |
dc.contributor.googleauthor | Jong-Wan Park | - |
dc.contributor.googleauthor | Hyowon Bang | - |
dc.contributor.googleauthor | Woo Kyung Kim | - |
dc.contributor.googleauthor | Yung E. Earm | - |
dc.contributor.googleauthor | Sung Joon Kim | - |
dc.identifier.doi | 10.1152/ajpcell.00052.2009 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01267 | - |
dc.relation.journalcode | J00102 | - |
dc.identifier.eissn | 1522-1563 | - |
dc.identifier.pmid | 19439530 | - |
dc.subject.keyword | K2P channel | - |
dc.subject.keyword | arachidonic acid | - |
dc.subject.keyword | PI kinase | - |
dc.subject.keyword | membrane stretch | - |
dc.subject.keyword | immune cells | - |
dc.contributor.alternativeName | Nam, Joo Hyun | - |
dc.contributor.affiliatedAuthor | Nam, Joo Hyun | - |
dc.citation.volume | 297 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 188 | - |
dc.citation.endPage | 197 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, Vol.297(1) : 188-197, 2009 | - |
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