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Kaempferol and quercetin, essential ingredients in Ginkgo biloba extract, inhibit interleukin-1beta-induced MUC5AC gene expression in human airway epithelial cells.

Authors
 Soon Ho Kwon  ;  Ji In Nam  ;  Si Hong Kim  ;  Jeong Hong Kim  ;  Joo-Heon Yoon  ;  Kyung-Su Kim 
Citation
 PHYTOTHERAPY RESEARCH, Vol.23(12) : 1708-1712, 2009 
Journal Title
 PHYTOTHERAPY RESEARCH 
ISSN
 0951-418X 
Issue Date
2009
MeSH
Cell Line ; Epithelial Cells/metabolism* ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Gene Expression/drug effects ; Ginkgo biloba/chemistry ; Humans ; Interleukin-1beta/pharmacology* ; Kaempferols/pharmacology* ; Mucin 5AC/genetics ; Mucin 5AC/metabolism* ; Phosphorylation ; Plant Extracts/pharmacology ; Quercetin/pharmacology* ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; p38 Mitogen-Activated Protein Kinases/metabolism
Keywords
Ginkgo bilobaextract ; MUC5AC ; kaempferol ; quercetin
Abstract
According to our previous study, Ginkgo biloba extract (GBE) suppresses IL-1beta-induced MUC5AC gene expression in NCI-H292 cells via the ERK and p38 MAPK pathways. This study sought to identify which ingredients of GBE suppress IL-1beta-induced MUC5AC gene expression in NCI-H292 cells and to examine which MAPKs are related to MUC5AC gene suppression for each ingredient. After the cells were pretreated with each ingredient and treated with IL-1beta (10 ng/mL), MUC5AC mRNA expression was determined by RT-PCR and real-time PCR. The results showed that kaempferol (KP) and quercetin (QC) suppressed MUC5AC mRNA expression in a dose-dependent manner, both with significant inhibition starting from 40 microm (equal concentration to about a twelfth or thirteenth dose of GBE). MAPK proteins were determined by western blot analysis after pretreatment with KP, QC and GBE. All three suppressed the phosphorylation of ERK and p38 kinases. In conclusion, the data suggested that KP and QC, essential ingredients in GBE, may overcome the dose problem of GBE and play a valuable role, clinically, in controlling mucin hypersecretion in airway inflammation.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/ptr.2817/abstract
DOI
10.1002/ptr.2817
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Soon Ho(권순호)
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Kim, Jeong Hong(김정홍)
Nam, Ji In(남지인)
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105477
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