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TGF-beta-induced interleukin-6 participates in transdifferentiation of human Tenon's fibroblasts to myofibroblasts

DC Field Value Language
dc.contributor.author김성주-
dc.contributor.author성공제-
dc.contributor.author이준행-
dc.contributor.author홍사민-
dc.date.accessioned2015-04-24T17:24:35Z-
dc.date.available2015-04-24T17:24:35Z-
dc.date.issued2009-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/105346-
dc.description.abstractPURPOSE: To gain a better understanding of the roles of interleukins (ILs) in subconjunctival fibrosis, we investigated their expression in transforming growth factor-beta1 (TGF-beta1)-stimulated Tenon's fibroblasts and examined their association with the transdifferentiation of fibroblasts to myofibroblasts. METHODS: After primary culture, fibroblasts derived from human Tenon's capsule were exposed to TGF-beta1. the expression of alpha-smooth muscle actin (alpha-SMA) protein was assessed by western immunoblots and immunofluorescence. the mRNA levels of various ILs were also evaluated by multiplex reverse transcription (RT)-PCR. Using the small interfering RNAs (siRNAs) specific for IL-6 and IL-11 and the promoter deletion assay, the contributions of IL-6 and IL-11 to TGF-beta1-induced induction of alpha-SMA were determined. RESULTS: in human Tenon's fibroblasts, TGF-beta1 stimulated the expression of alpha-SMA protein determined by western blot analysis and also increased the mRNA levels of IL-6 and IL-11 determined by multiplex RT-PCR. on the western immunoblots and immunofluorescence, the increased expression of alpha-SMA was attenuated only by the siRNAs specific for IL-6 but not by the siRNAs specific for IL-11. When the activator protein-1 binding sites of the IL-6 promoter region were deleted, the stimulation effects of TGF-beta1 decreased. CONCLUSIONS: Our data show that autocrine IL-6 may participate in the TGF-beta1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts, which is known to be an essential step for subconjunctival fibrosis.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2123~2128-
dc.relation.isPartOfMOLECULAR VISION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActins/metabolism-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Transdifferentiation/drug effects*-
dc.subject.MESHFibroblasts/cytology*-
dc.subject.MESHFibroblasts/drug effects*-
dc.subject.MESHFibroblasts/metabolism-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-11/genetics-
dc.subject.MESHInterleukin-11/metabolism-
dc.subject.MESHInterleukin-6/genetics-
dc.subject.MESHInterleukin-6/metabolism*-
dc.subject.MESHPromoter Regions, Genetic/genetics-
dc.subject.MESHRNA Interference/drug effects-
dc.subject.MESHRNA, Small Interfering/metabolism-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSequence Deletion-
dc.subject.MESHTranscription Factor AP-1/metabolism-
dc.subject.MESHTransforming Growth Factor beta/pharmacology*-
dc.subject.MESHUp-Regulation/drug effects-
dc.titleTGF-beta-induced interleukin-6 participates in transdifferentiation of human Tenon's fibroblasts to myofibroblasts-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorGong Je Seong-
dc.contributor.googleauthorSamin Hong-
dc.contributor.googleauthorSun-Ah Jung-
dc.contributor.googleauthorJung-Jin Lee-
dc.contributor.googleauthorEunhae Lim-
dc.contributor.googleauthorSung-Joo Kim-
dc.contributor.googleauthorJoon H. Lee-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00584-
dc.contributor.localIdA01946-
dc.contributor.localIdA03180-
dc.contributor.localIdA04395-
dc.relation.journalcodeJ02272-
dc.identifier.eissn1090-0535-
dc.identifier.pmid19862334-
dc.contributor.alternativeNameKim, Sung Joo-
dc.contributor.alternativeNameSeong, Gong Je-
dc.contributor.alternativeNameLee, Joon Haeng-
dc.contributor.alternativeNameHong, Sa Min-
dc.contributor.affiliatedAuthorKim, Sung Joo-
dc.contributor.affiliatedAuthorSeong, Gong Je-
dc.contributor.affiliatedAuthorLee, Joon Haeng-
dc.contributor.affiliatedAuthorHong, Sa Min-
dc.citation.volume15-
dc.citation.startPage2123-
dc.citation.endPage2128-
dc.identifier.bibliographicCitationMOLECULAR VISION, Vol.15 : 2123-2128, 2009-
dc.identifier.rimsid54720-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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