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TGF-beta-induced interleukin-6 participates in transdifferentiation of human Tenon's fibroblasts to myofibroblasts
DC Field | Value | Language |
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dc.contributor.author | 김성주 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 이준행 | - |
dc.contributor.author | 홍사민 | - |
dc.date.accessioned | 2015-04-24T17:24:35Z | - |
dc.date.available | 2015-04-24T17:24:35Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105346 | - |
dc.description.abstract | PURPOSE: To gain a better understanding of the roles of interleukins (ILs) in subconjunctival fibrosis, we investigated their expression in transforming growth factor-beta1 (TGF-beta1)-stimulated Tenon's fibroblasts and examined their association with the transdifferentiation of fibroblasts to myofibroblasts. METHODS: After primary culture, fibroblasts derived from human Tenon's capsule were exposed to TGF-beta1. the expression of alpha-smooth muscle actin (alpha-SMA) protein was assessed by western immunoblots and immunofluorescence. the mRNA levels of various ILs were also evaluated by multiplex reverse transcription (RT)-PCR. Using the small interfering RNAs (siRNAs) specific for IL-6 and IL-11 and the promoter deletion assay, the contributions of IL-6 and IL-11 to TGF-beta1-induced induction of alpha-SMA were determined. RESULTS: in human Tenon's fibroblasts, TGF-beta1 stimulated the expression of alpha-SMA protein determined by western blot analysis and also increased the mRNA levels of IL-6 and IL-11 determined by multiplex RT-PCR. on the western immunoblots and immunofluorescence, the increased expression of alpha-SMA was attenuated only by the siRNAs specific for IL-6 but not by the siRNAs specific for IL-11. When the activator protein-1 binding sites of the IL-6 promoter region were deleted, the stimulation effects of TGF-beta1 decreased. CONCLUSIONS: Our data show that autocrine IL-6 may participate in the TGF-beta1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts, which is known to be an essential step for subconjunctival fibrosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2123~2128 | - |
dc.relation.isPartOf | MOLECULAR VISION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Actins/metabolism | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Transdifferentiation/drug effects* | - |
dc.subject.MESH | Fibroblasts/cytology* | - |
dc.subject.MESH | Fibroblasts/drug effects* | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Fluorescent Antibody Technique | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-11/genetics | - |
dc.subject.MESH | Interleukin-11/metabolism | - |
dc.subject.MESH | Interleukin-6/genetics | - |
dc.subject.MESH | Interleukin-6/metabolism* | - |
dc.subject.MESH | Promoter Regions, Genetic/genetics | - |
dc.subject.MESH | RNA Interference/drug effects | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Sequence Deletion | - |
dc.subject.MESH | Transcription Factor AP-1/metabolism | - |
dc.subject.MESH | Transforming Growth Factor beta/pharmacology* | - |
dc.subject.MESH | Up-Regulation/drug effects | - |
dc.title | TGF-beta-induced interleukin-6 participates in transdifferentiation of human Tenon's fibroblasts to myofibroblasts | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.contributor.googleauthor | Samin Hong | - |
dc.contributor.googleauthor | Sun-Ah Jung | - |
dc.contributor.googleauthor | Jung-Jin Lee | - |
dc.contributor.googleauthor | Eunhae Lim | - |
dc.contributor.googleauthor | Sung-Joo Kim | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00584 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A03180 | - |
dc.contributor.localId | A04395 | - |
dc.relation.journalcode | J02272 | - |
dc.identifier.eissn | 1090-0535 | - |
dc.identifier.pmid | 19862334 | - |
dc.contributor.alternativeName | Kim, Sung Joo | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Lee, Joon Haeng | - |
dc.contributor.alternativeName | Hong, Sa Min | - |
dc.contributor.affiliatedAuthor | Kim, Sung Joo | - |
dc.contributor.affiliatedAuthor | Seong, Gong Je | - |
dc.contributor.affiliatedAuthor | Lee, Joon Haeng | - |
dc.contributor.affiliatedAuthor | Hong, Sa Min | - |
dc.citation.volume | 15 | - |
dc.citation.startPage | 2123 | - |
dc.citation.endPage | 2128 | - |
dc.identifier.bibliographicCitation | MOLECULAR VISION, Vol.15 : 2123-2128, 2009 | - |
dc.identifier.rimsid | 54720 | - |
dc.type.rims | ART | - |
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