Cited 23 times in
Agonist-induced internalization of mGluR1alpha is mediated by caveolin.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철훈 | - |
dc.date.accessioned | 2015-04-24T17:24:19Z | - |
dc.date.available | 2015-04-24T17:24:19Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105338 | - |
dc.description.abstract | Agonist-induced internalization of metabotropic glutamate receptors (mGluRs) plays an important role in neuronal signaling. Although internalization of mGluRs has been reported to be mediated by clathrin-dependent pathway, studies describing clathrin-independent pathways are emerging. Here, we report that agonist-induced internalization of mGluR1alpha is mediated by caveolin. We show that two caveolin-binding motifs of mGluR1alpha interact with caveolin1/2. Using cell surface-immunoprecipitation and total internal reflection fluorescence imaging, we found that agonist-induced internalization of mGluR1alpha is regulated by caveolin-binding motifs of the receptor in heterologous cells. Moreover, in the cerebellum, group I mGluR agonist dihydroxyphenylglycol increased the interaction of phosphorylated caveolin with mGluR1alpha. This interaction was blocked by methyl-beta-cyclodextrin, known to disrupt caveolin/caveolae-dependent signaling by cholesterol depletion. Methyl-beta-cyclodextrin also blocked the agonist-induced internalization of mGluR1alpha. Thus, these findings represent the evidence for agonist-induced internalization of mGluR1alpha via caveolin and suggest that caveolin might play a role in synaptic metaplasticity by regulating internalization of mGluR1alpha in the cerebellum. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 61~71 | - |
dc.relation.isPartOf | JOURNAL OF NEUROCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Carcinoma | - |
dc.subject.MESH | Caveolins/metabolism* | - |
dc.subject.MESH | Cell Line, Transformed | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cerebellum/cytology | - |
dc.subject.MESH | Cerebellum/drug effects | - |
dc.subject.MESH | Cerebellum/metabolism | - |
dc.subject.MESH | Endocytosis/drug effects* | - |
dc.subject.MESH | Endocytosis/physiology* | - |
dc.subject.MESH | Excitatory Amino Acid Agonists/pharmacology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoprecipitation/methods | - |
dc.subject.MESH | In Vitro Techniques | - |
dc.subject.MESH | Intracellular Fluid/drug effects | - |
dc.subject.MESH | Intracellular Fluid/metabolism | - |
dc.subject.MESH | Luminescent Proteins/genetics | - |
dc.subject.MESH | Membrane Microdomains/drug effects | - |
dc.subject.MESH | Membrane Microdomains/metabolism | - |
dc.subject.MESH | Mutation/genetics | - |
dc.subject.MESH | Quisqualic Acid/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Receptors, Metabotropic Glutamate/genetics | - |
dc.subject.MESH | Receptors, Metabotropic Glutamate/metabolism* | - |
dc.subject.MESH | Transfection/methods | - |
dc.title | Agonist-induced internalization of mGluR1alpha is mediated by caveolin. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Yun Hwa Hong | - |
dc.contributor.googleauthor | Ji Young Kim | - |
dc.contributor.googleauthor | Jeong Ho Lee | - |
dc.contributor.googleauthor | Hong Gu Chae | - |
dc.contributor.googleauthor | Sung Soo Jang | - |
dc.contributor.googleauthor | Ju Hong Jeon | - |
dc.contributor.googleauthor | Chul Hoon Kim | - |
dc.contributor.googleauthor | Jun Kim | - |
dc.contributor.googleauthor | Sang Jeong Kim | - |
dc.identifier.doi | 10.1111/j.1471-4159.2009.06289.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01057 | - |
dc.relation.journalcode | J01620 | - |
dc.identifier.eissn | 1471-4159 | - |
dc.identifier.pmid | 19627451 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2009.06289.x/abstract | - |
dc.subject.keyword | agonist-induced internalization | - |
dc.subject.keyword | caveolin | - |
dc.subject.keyword | cerebellum | - |
dc.subject.keyword | mGluR1[alpha] | - |
dc.contributor.alternativeName | Kim, Chul Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Chul Hoon | - |
dc.citation.volume | 111 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 61 | - |
dc.citation.endPage | 71 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROCHEMISTRY, Vol.111(1) : 61-71, 2009 | - |
dc.identifier.rimsid | 54714 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.