394 424

Cited 114 times in

Adiponectin stimulates osteoblast differentiation through induction of COX2 in mesenchymal progenitor cells

Authors
 HYUN WOO LEE  ;  SANG YUN KIM  ;  A YOUNG KIM  ;  EUN JIG LEE  ;  JE-YONG CHOI  ;  JAE BUM KIM 
Citation
 STEM CELLS, Vol.27(9) : 2254-2262, 2009 
Journal Title
STEM CELLS
ISSN
 1066-5099 
Issue Date
2009
MeSH
Adiponectin/pharmacology* ; Animals ; Blotting, Western ; Bone Morphogenetic Protein 2/genetics ; Bone Morphogenetic Protein 2/metabolism ; Cell Differentiation*/drug effects ; Cell Line ; Chromatin Immunoprecipitation ; Cyclooxygenase 2/metabolism ; Cyclooxygenase 2/physiology* ; Enzyme Activators/pharmacology ; Humans ; Immunoenzyme Techniques ; Mesenchymal Stromal Cells/cytology* ; Mesenchymal Stromal Cells/drug effects* ; Mice ; Osteoblasts/cytology* ; Osteogenesis/drug effects ; Polymerase Chain Reaction ; Proto-Oncogene Proteins c-jun/genetics ; Proto-Oncogene Proteins c-jun/physiology ; RNA, Small Interfering ; p38 Mitogen-Activated Protein Kinases/metabolism
Keywords
Adiponectin ; Mesenchymal progenitor cell ; Osteogenesis ; COX2 ; c-Jun
Abstract
In bone marrow, osteoblasts and adipocytes are differentiated from mesenchymal progenitor cells and their differentiation is reciprocally regulated by largely unknown mechanisms. In this study, we investigated downstream signaling cascades of adiponectin, a member of the adipocytokine family, in the regulation of osteoblast differentiation. Adiponectin augmented expression of several osteogenic marker genes and increased osteoblast differentiation in mesenchymal progenitor cells. The expression of cyclooxygenase-2 (COX2) was potently increased by adiponectin, whereas inhibition of COX2 activity abolished the effect of adiponectin on osteogenesis. In addition, adiponectin rapidly stimulated p38 mitogen-activated protein kinase via the adiponectin receptor, AdipoR1, which resulted in c-Jun activation for COX2 expression. Adiponectin also stimulated BMP2 expression in a COX2-dependent manner. Moreover, Runx2, a key osteogenic transcription factor, contributed to the acceleration of osteogenesis in the presence of adiponectin. Collectively, the finding that adiponectin could promote osteogenesis through an intracellular signaling cascade in mesenchymal progenitor cells suggests that adiponectin would be a potential therapeutic target for bone-related diseases.
Files in This Item:
T200903469.pdf Download
DOI
10.1002/stem.144
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105059
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links