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Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced by Entamoeba histolytica

Authors
 K. A. KIM  ;  Y. A. LEE  ;  M. H. SHIN 
Citation
 PARASITE IMMUNOLOGY, Vol.32(3) : 176-83, 2009 
Journal Title
 PARASITE IMMUNOLOGY 
ISSN
 0141-9838 
Issue Date
2009
MeSH
Arsenicals/pharmacology ; Calpain/antagonists & inhibitors ; Calpain/metabolism* ; Cell Death ; Dipeptides/pharmacology ; Entamoeba histolytica/immunology* ; Entamoeba histolytica/pathogenicity ; Enzyme Inhibitors/pharmacology ; Humans ; Jurkat Cells ; Protein Tyrosine Phosphatase, Non-Receptor Type 11/metabolism* ; Protein Tyrosine Phosphatase, Non-Receptor Type 6/metabolism* ; T-Lymphocytes/metabolism* ; T-Lymphocytes/parasitology*
Keywords
calpain ; cell death ; dephosphorylation ; Entamoeba histolytica ; host cell ; tyrosine phosphatase SHP-1 and SHP-2
Abstract
Host cell death induced by Entamoeba histolytica is an important mechanism for both host defence and microbial immune evasion during human amoebiasis. However, the signalling pathways underlying cell death induced by E. histolytica are not fully understood. This study investigated the involvement of the protein tyrosine phosphatases (PTPs) SHP-1 and SHP-2 in the dephosphorylation associated with E. histolytica-induced host cell death. Incubation with E. histolytica resulted in a marked decrease in protein tyrosine phosphorylation levels and degradation of SHP-1 or SHP-2 in Jurkat cells. Pre-treatment of cells with a calpain inhibitor, calpeptin, impeded the amoeba-induced dephosporylation and cleavage of SHP-1 or SHP-2. Additionally, inhibition of PTPs with phenylarsine oxide (PAO) attenuated Entamoeba-induced dephosphorylation and DNA fragmentation in Jurkat T cells. These results suggest that calpain-dependent cleavage of SHP-1 and SHP-2 may contribute to protein tyrosine dephosphorylation in Jurkat T cell death induced by E. histolytica.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3024.2009.01175.x/abstract
DOI
10.1111/j.1365-3024.2009.01175.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Ah(김경아)
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105031
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