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Functional and molecular expression of epithelial sodium channels in cultured human endolymphatic sac epithelial cells.

 Sung Huhn Kim  ;  Hun Yi Park  ;  Hyun Seung Choi  ;  Hyun Pil Chung  ;  Jae Young Choi 
 OTOLOGY & NEUROTOLOGY, Vol.30(4) : 529-534, 2009 
Journal Title
Issue Date
Cell Culture Techniques ; Cell Differentiation ; Endolymphatic Sac*/cytology ; Endolymphatic Sac*/physiology ; Epithelial Cells/cytology ; Epithelial Cells/metabolism* ; Epithelial Sodium Channels/biosynthesis* ; Gene Expression/drug effects ; Humans ; Immunohistochemistry ; Interleukin-1beta/administration & dosage ; Membrane Potentials/drug effects ; Mitochondria/metabolism ; Protein Isoforms/biosynthesis ; RNA, Messenger/analysis ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Ribosomes/metabolism
HYPOTHESIS: Epithelial sodium channels are expressed in cultured human endolymphatic sac (ES) epithelial (HESE) cells and epithelial sodium channel (ENaC) expression is suppressed by interleukin 1beta. BACKGROUND: The ES is part of the membranous labyrinth in the inner ear that plays an important role in maintaining homeostasis of the endolymphatic fluid system. However, the exact mechanism of fluid volume regulation is not yet known. METHODS: The ES specimens were harvested during acoustic neuroma surgery (n = 13) using the translabyrinthine approach and were subcultured with high-epidermal growth factor (25 ng/mL) media. RESULTS: The serially passaged HESE cells differentiated into a monolayer of confluent cells and some of the cultured cells had features of mitochondria-rich cells. Reverse transcription-polymerase chain reaction revealed that ENaC subunits are expressed in the cultured HESE cells. We also confirmed the presence of an ENaC-dependent short-circuit current in the cultured HESE cells. Interestingly, ENaC mRNA expression and ENaC-dependent current decreased after treatment with interleukin 1beta (10 nmol/L for 24 h). CONCLUSION: These findings suggest that ENaC plays an important role in fluid absorption in the human ES and that its function may be altered during inflammatory conditions.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Huhn(김성헌)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
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