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Human plasma carboxylesterase 1, a novel serologic biomarker candidate for hepatocellular carcinoma

 Keun Na  ;  Eun-Young Lee  ;  Hyoung-Joo Lee  ;  Kwang-Youl Kim  ;  Hanna Lee  ;  Seul-Ki Jeong  ;  An-Sung Jeong  ;  Sang Yun Cho  ;  Sun A. Kim  ;  Si Young Song  ;  Kyung Sik Kim  ;  Sung Won Cho  ;  Hoguen Kim  ;  Young-Ki Paik 
 PROTEOMICS, Vol.9(16) : 3989-3999, 2009 
Journal Title
Issue Date
Biomarkers, Tumor/blood* ; Blotting, Western ; Carboxylic Ester Hydrolases/blood* ; Carboxylic Ester Hydrolases/metabolism* ; Carcinoma, Hepatocellular/blood* ; Carcinoma, Hepatocellular/enzymology* ; Chromatography, Liquid ; Electrophoresis, Gel, Two-Dimensional ; Glycoproteins/blood ; Humans ; Immunohistochemistry ; Immunoprecipitation ; In Vitro Techniques ; Liver Neoplasms/blood* ; Liver Neoplasms/enzymology* ; Reverse Transcriptase Polymerase Chain Reaction ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tandem Mass Spectrometry
Biomarker ; Carboxylesterase 1 ; Glycoproteomics ; Hepatocellular carcinoma ; N‐linked glycoprotein
To identify and characterize a serologic glycoprotein biomarker for hepatocellular carcinoma (HCC), multi-lectin affinity chromatography was used to isolate intracellular N-linked glycoprotein fractions from five paired non-tumor and tumor tissues. From the series of 2-D DIGE targeted differentially expressed N-linked glycoproteins, we identified human liver carboxylesterase 1 (hCE1), which was remarkably down-regulated in tumor tissues, a finding confirmed by Western blot, a quantitative real-time RT-PCR, and immunohistochemical staining of non-tumor and tumor tissues from total 58 HCC patients. To investigate whether hCE1 is also present in human plasma, we employed a magnetic bead-based immunoprecipitation followed by nano-LC-MS/MS analysis, and we found for the first time that hCE1 is present in human plasma as opposed to that in liver tissues. That is, from normalization of hCE1 signal by the immunoprecipitation and Western blot analysis, hCE1 levels were increased in plasma specimens from HCC patients than in plasma from other disease patient groups (e.g. liver cirrhosis, chronic hepatitis, cholangiocarcinoma, stomach cancer, and pancreatic cancer). From the receiver operating characteristic analysis in HCC, both sensitivity and specificity were shown to be greater than 70.0 and 85.0%, respectively. Thus, the high-resolution proteomic approach demonstrates that hCE1 is a good candidate for further validation as a serologic glycoprotein biomarker for HCC.
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Kim, Sun A(김선아) ORCID logo https://orcid.org/0000-0002-7174-3640
Kim, Hogeun(김호근)
Song, Si Young(송시영) ORCID logo https://orcid.org/0000-0002-1417-4314
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