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Identification of HLA-A*2402-restricted HCMV immediate early-1 (IE-1) epitopes as targets for CD8+ HCMV-specific cytotoxic T lymphocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김현옥 | - |
dc.contributor.author | 이경원 | - |
dc.contributor.author | 임종백 | - |
dc.contributor.author | 장선필 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 이상국 | - |
dc.date.accessioned | 2015-04-24T17:00:12Z | - |
dc.date.available | 2015-04-24T17:00:12Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104577 | - |
dc.description.abstract | BACKGROUND: To identify novel HLA-A*2402-restricted human cytomegalovirus (HCMV) immediate early-1 (IE-1) epitopes for adoptive immunotherapy, we explored 120 overlapping 15-amino acid spanning IE-1. METHODS: These peptides were screened by measuring the frequency of polyclonal CD8+ T cells producing intracellular interferon-gamma (IFN-gamma) using flow cytometry and the epitopes were validated with a HCMV-infected target Cr release cytotoxicity assay. RESULTS: Initial screening was performed with 12 mini-pools of 10 consecutive peptides made from 120 overlapping peptides15-amino acids in length that spanned IE-1. When peripheral blood mononuclear cells (PBMCs) from HLA-A*2402 HCMV-seropositive donors were sensitized with each of the 12 mini-pools, mini-pools 1 and 2 induced the highest frequency of CD8+ cytotoxic T lymphocytes (CTLs) producing IFN-gamma. When PBMCs were stimulated with each of the twenty peptides belonging to mini-pools 1 and 2, peptides IE-11-15MESSAKRKMDPDNPD and IE-15-19AKRKMDPDNPDEGPS induced the greatest quantities of IFN-gamma production and cytotoxicity of HLA-matched HCMV-infected fibroblasts. To determine the exact HLA-A*2402-restricted epitopes within the two IE-1 proteins, we synthesized a total of twenty-one overlapping 9- or 10 amino acid peptides spanning IE-11-15 and IE-15-19. Peptide IE-13-12SSAKRKMDPD induced the greatest quantities of IFN-gamma production and target cell killing by CD8+ CTLs. CONCLUSION: HCMV IE-13-12SSAKRKMDPD is a HLA-A*2402-restricted HCMV IE-1 epitope that can serve as a common target for CD8+ HCMV-specific CTLs. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | JOURNAL OF TRANSLATIONAL MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | CD8-Positive T-Lymphocytes/immunology* | - |
dc.subject.MESH | CD8-Positive T-Lymphocytes/virology | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cytomegalovirus/immunology* | - |
dc.subject.MESH | Cytomegalovirus Infections/immunology | - |
dc.subject.MESH | Cytomegalovirus Infections/therapy | - |
dc.subject.MESH | Epitopes/genetics | - |
dc.subject.MESH | Epitopes/immunology* | - |
dc.subject.MESH | Fibroblasts/cytology | - |
dc.subject.MESH | Fibroblasts/immunology | - |
dc.subject.MESH | HLA-A Antigens/immunology* | - |
dc.subject.MESH | HLA-A24 Antigen | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immediate-Early Proteins/genetics | - |
dc.subject.MESH | Immediate-Early Proteins/immunology* | - |
dc.subject.MESH | Interferon-gamma/immunology | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Peptides/genetics | - |
dc.subject.MESH | Peptides/immunology | - |
dc.subject.MESH | T-Lymphocyte Subsets/immunology* | - |
dc.subject.MESH | T-Lymphocyte Subsets/virology | - |
dc.subject.MESH | T-Lymphocytes, Cytotoxic/immunology* | - |
dc.subject.MESH | T-Lymphocytes, Cytotoxic/virology | - |
dc.subject.MESH | Viral Proteins/genetics | - |
dc.subject.MESH | Viral Proteins/immunology | - |
dc.title | Identification of HLA-A*2402-restricted HCMV immediate early-1 (IE-1) epitopes as targets for CD8+ HCMV-specific cytotoxic T lymphocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Jong-Baeck Lim | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Joo Eun Ha | - |
dc.contributor.googleauthor | Sunphil Jang | - |
dc.contributor.googleauthor | Sang-Guk Lee | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | David Stroncek | - |
dc.identifier.doi | 10.1186/1479-5876-7-72 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01122 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A03403 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A03433 | - |
dc.relation.journalcode | J01915 | - |
dc.identifier.eissn | 1479-5876 | - |
dc.identifier.pmid | 19698161 | - |
dc.subject.keyword | HCMV Infection | - |
dc.subject.keyword | Adoptive Immunotherapy | - |
dc.subject.keyword | Immunogenic Epitope | - |
dc.subject.keyword | Pepro Tech | - |
dc.subject.keyword | HCMV Disease | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Lim, Jong Baeck | - |
dc.contributor.alternativeName | Jang, Sun Phil | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lim, Jong Baeck | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Jang, Sun Phil | - |
dc.citation.volume | 7 | - |
dc.citation.startPage | 72 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TRANSLATIONAL MEDICINE, Vol.7 : 72, 2009 | - |
dc.identifier.rimsid | 51120 | - |
dc.type.rims | ART | - |
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