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Identification of HLA-A*2402-restricted HCMV immediate early-1 (IE-1) epitopes as targets for CD8+ HCMV-specific cytotoxic T lymphocytes

DC FieldValueLanguage
dc.contributor.author김현옥-
dc.contributor.author이경원-
dc.contributor.author임종백-
dc.contributor.author장선필-
dc.contributor.author정석훈-
dc.contributor.author이상국-
dc.date.accessioned2015-04-24T17:00:12Z-
dc.date.available2015-04-24T17:00:12Z-
dc.date.issued2009-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104577-
dc.description.abstractBACKGROUND: To identify novel HLA-A*2402-restricted human cytomegalovirus (HCMV) immediate early-1 (IE-1) epitopes for adoptive immunotherapy, we explored 120 overlapping 15-amino acid spanning IE-1. METHODS: These peptides were screened by measuring the frequency of polyclonal CD8+ T cells producing intracellular interferon-gamma (IFN-gamma) using flow cytometry and the epitopes were validated with a HCMV-infected target Cr release cytotoxicity assay. RESULTS: Initial screening was performed with 12 mini-pools of 10 consecutive peptides made from 120 overlapping peptides15-amino acids in length that spanned IE-1. When peripheral blood mononuclear cells (PBMCs) from HLA-A*2402 HCMV-seropositive donors were sensitized with each of the 12 mini-pools, mini-pools 1 and 2 induced the highest frequency of CD8+ cytotoxic T lymphocytes (CTLs) producing IFN-gamma. When PBMCs were stimulated with each of the twenty peptides belonging to mini-pools 1 and 2, peptides IE-11-15MESSAKRKMDPDNPD and IE-15-19AKRKMDPDNPDEGPS induced the greatest quantities of IFN-gamma production and cytotoxicity of HLA-matched HCMV-infected fibroblasts. To determine the exact HLA-A*2402-restricted epitopes within the two IE-1 proteins, we synthesized a total of twenty-one overlapping 9- or 10 amino acid peptides spanning IE-11-15 and IE-15-19. Peptide IE-13-12SSAKRKMDPD induced the greatest quantities of IFN-gamma production and target cell killing by CD8+ CTLs. CONCLUSION: HCMV IE-13-12SSAKRKMDPD is a HLA-A*2402-restricted HCMV IE-1 epitope that can serve as a common target for CD8+ HCMV-specific CTLs.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfJOURNAL OF TRANSLATIONAL MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHCD8-Positive T-Lymphocytes/immunology*-
dc.subject.MESHCD8-Positive T-Lymphocytes/virology-
dc.subject.MESHCell Line-
dc.subject.MESHCytomegalovirus/immunology*-
dc.subject.MESHCytomegalovirus Infections/immunology-
dc.subject.MESHCytomegalovirus Infections/therapy-
dc.subject.MESHEpitopes/genetics-
dc.subject.MESHEpitopes/immunology*-
dc.subject.MESHFibroblasts/cytology-
dc.subject.MESHFibroblasts/immunology-
dc.subject.MESHHLA-A Antigens/immunology*-
dc.subject.MESHHLA-A24 Antigen-
dc.subject.MESHHumans-
dc.subject.MESHImmediate-Early Proteins/genetics-
dc.subject.MESHImmediate-Early Proteins/immunology*-
dc.subject.MESHInterferon-gamma/immunology-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPeptides/genetics-
dc.subject.MESHPeptides/immunology-
dc.subject.MESHT-Lymphocyte Subsets/immunology*-
dc.subject.MESHT-Lymphocyte Subsets/virology-
dc.subject.MESHT-Lymphocytes, Cytotoxic/immunology*-
dc.subject.MESHT-Lymphocytes, Cytotoxic/virology-
dc.subject.MESHViral Proteins/genetics-
dc.subject.MESHViral Proteins/immunology-
dc.titleIdentification of HLA-A*2402-restricted HCMV immediate early-1 (IE-1) epitopes as targets for CD8+ HCMV-specific cytotoxic T lymphocytes-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorJong-Baeck Lim-
dc.contributor.googleauthorHyun Ok Kim-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorJoo Eun Ha-
dc.contributor.googleauthorSunphil Jang-
dc.contributor.googleauthorSang-Guk Lee-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorDavid Stroncek-
dc.identifier.doi10.1186/1479-5876-7-72-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01122-
dc.contributor.localIdA02649-
dc.contributor.localIdA03403-
dc.contributor.localIdA03619-
dc.contributor.localIdA03433-
dc.relation.journalcodeJ01915-
dc.identifier.eissn1479-5876-
dc.identifier.pmid19698161-
dc.subject.keywordHCMV Infection-
dc.subject.keywordAdoptive Immunotherapy-
dc.subject.keywordImmunogenic Epitope-
dc.subject.keywordPepro Tech-
dc.subject.keywordHCMV Disease-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameLim, Jong Baeck-
dc.contributor.alternativeNameJang, Sun Phil-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorLim, Jong Baeck-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorJang, Sun Phil-
dc.citation.volume7-
dc.citation.startPage72-
dc.identifier.bibliographicCitationJOURNAL OF TRANSLATIONAL MEDICINE, Vol.7 : 72, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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