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Direct reprogramming of human neural stem cells by OCT4.

Authors
 Jeong Beom Kim  ;  Boris Greber  ;  Marcos J. Arau´zo-Bravo  ;  Johann Meyer  ;  Kook In Park  ;  Holm Zaehres  ;  Hans R. Scho¨ler 
Citation
 Nature, Vol.461(7264) : 649-653, 2009 
Journal Title
 Nature 
ISSN
 0028-0836 
Issue Date
2009
MeSH
Animals ; Biomarkers/analysis ; Cell Dedifferentiation* ; Cell Differentiation ; Cell Line ; Cellular Reprogramming* ; DNA Methylation ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Epigenesis, Genetic ; Fetus/cytology* ; Gene Expression Profiling ; Germ Layers/cytology ; Germ Layers/metabolism ; Humans ; Mice ; Neurons/cytology* ; Neurons/metabolism ; Octamer Transcription Factor-3/genetics ; Octamer Transcription Factor-3/metabolism* ; Pluripotent Stem Cells/cytology* ; Pluripotent Stem Cells/metabolism*
Abstract
Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.
Full Text
http://www.nature.com/nature/journal/v461/n7264/full/nature08436.html
DOI
10.1038/nature08436
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Park, Kook In(박국인) ORCID logo https://orcid.org/0000-0001-8499-9293
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104519
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