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The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth

DC FieldValueLanguage
dc.contributor.author김경환-
dc.contributor.author김주영-
dc.contributor.author이민구-
dc.date.accessioned2015-04-24T16:56:08Z-
dc.date.available2015-04-24T16:56:08Z-
dc.date.issued2009-
dc.identifier.issn0023-6837-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104448-
dc.description.abstractThe formation of a pH gradient, which is characterized by intracellular alkalinization and extracellular acidification, plays a key role in the growth and metastasis of tumor cells. However, the underlying mechanisms of alkalinization-induced cell growth are not known. In this study, we investigated the roles of eukaryotic translation elongation factor 1 alpha (eEF1A) in alkalinization-induced cell growth. In all cell lines tested (NIH3T3, HEK293, and HeLa), cell growth was affected by the modulation of intracellular pH. In general, weak intracellular alkalinization produced increased cell growth, whereas intracellular acidification resulted in decreased cell growth. It is interesting to note that portions of actin-bound eEF1A proteins were gradually reduced from acidic to alkaline conditions, suggesting an increase in levels of functionally active, free-form eEF1A. Over-expression of eEF1A caused increased cell growth in HeLa cells. It should be noted that dissociation of eEF1A from actin by transfection with the actin-binding domain deleted eEF1A construct further increased cell growth under acidic conditions, whereas most of the intact eEF1A was bound to actin. Conversely, knockdown of eEF1A by treatment with eEF1A1 and eEF1A2 siRNAs nullified the effects of alkalinization-induced cell growth. The above findings suggest that an increase in free-form eEF1A under alkaline conditions plays a critical role in alkalinization-induced cell growth.-
dc.description.statementOfResponsibilityopen-
dc.format.extent867~874-
dc.relation.isPartOfLABORATORY INVESTIGATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcid-Base Equilibrium/physiology*-
dc.subject.MESHAnimals-
dc.subject.MESHCarbon Dioxide/pharmacology*-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHumans-
dc.subject.MESHHydrogen-Ion Concentration-
dc.subject.MESHMice-
dc.subject.MESHNIH 3T3 Cells-
dc.subject.MESHPeptide Elongation Factor 1/genetics-
dc.subject.MESHPeptide Elongation Factor 1/metabolism*-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHUterine Cervical Neoplasms/drug therapy-
dc.subject.MESHUterine Cervical Neoplasms/metabolism-
dc.subject.MESHUterine Cervical Neoplasms/pathology*-
dc.titleThe role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJuno Kim-
dc.contributor.googleauthorWan Namkung-
dc.contributor.googleauthorJae Seok Yoon-
dc.contributor.googleauthorMin Jae Jo-
dc.contributor.googleauthorSung Hee Lee-
dc.contributor.googleauthorKyung Hwan Kim-
dc.contributor.googleauthorJoo Young Kim-
dc.contributor.googleauthorMin Goo Lee-
dc.identifier.doi10.1038/labinvest.2009.53-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00942-
dc.contributor.localIdA00311-
dc.contributor.localIdA02781-
dc.relation.journalcodeJ02150-
dc.identifier.eissn1530-0307-
dc.identifier.pmid19506553-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.alternativeNameKim, Joo Young-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthorKim, Joo Young-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.citation.volume89-
dc.citation.number8-
dc.citation.startPage867-
dc.citation.endPage874-
dc.identifier.bibliographicCitationLABORATORY INVESTIGATION, Vol.89(8) : 867-874, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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