Population structure and distribution of virulence-related genes of Bacteroides fragilis isolates from Korea and Japan

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Citation: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.64(3) : 340-343, 2009

MeSH: Bacterial Proteins/genetics ; Bacteroides Infections/microbiology* ; Bacteroides fragilis/classification* ; Bacteroides fragilis/genetics ; Bacteroides fragilis/isolation & purification* ; Cluster Analysis ; DNA, Bacterial/genetics* ; Genotype ; Humans ; Japan ; Korea ; Phylogeny ; Polymorphism, Genetic* ; Recombination, Genetic ; Sequence Analysis, DNA ; Virulence Factors/genetics*

Abstract: Sequences for rpoB, gyrB, pdiA, and ompA were determined from 63 Bacteroides fragilis isolates, which were from Korea and Japan and include 4 reference strains. All 4 gene sequences supported clear separation of the cfi(+) group from the cfi(-) group. Combined sequences of the 60 division I isolates (cfi(-)) produced 45 different clones. Apparent discordance of gene trees, index of association, maximum likelihood test, and homoplasy ratio all supported a high frequency of recombination. There was no association between the presence of virulence-related genes and phylogenetic clustering in any gene tree