2 871

Cited 24 times in

High-throughput identification of clinically important bacterial pathogens using DNA microarray

DC Field Value Language
dc.contributor.author금기창-
dc.contributor.author김준명-
dc.contributor.author유내춘-
dc.contributor.author유원민-
dc.contributor.author장경희-
dc.contributor.author최준용-
dc.date.accessioned2015-04-24T16:54:52Z-
dc.date.available2015-04-24T16:54:52Z-
dc.date.issued2009-
dc.identifier.issn0890-8508-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/104409-
dc.description.abstractRapid and accurate detection of pathogenic bacteria is important for the treatment of patients with suitable antibiotics. Here we report the development of a diagnostic DNA microarray for the high-throughput identification of 39 pathogenic bacteria selected based on their high prevalence rate and/or difficulty of cultivation. the 23S ribosomal DNA and 16S-23S rDNA intergenic spacer region were used as target DNAs for pathogen detection. Universal- and species-specific probes were designed based on the unique and common sites within the target DNA sequences. New target DNA sequences were determined for the detection of 19 bacterial pathogens. the usefulness of the designed probes was validated using 39 reference bacteria and also with 515 clinical isolates from various clinical samples including blood, stool, pus, sputum, urine and cerebrospinal fluid. the DNA microarray developed in this study allowed efficient detection of bacterial pathogens with the specificities of 100%. the sensitivities were 100% as well except for the two pathogens, Enterobacter cloacae (75%) and Enterococcus faecium (85%). These results suggest that the DNA microarray-based assay developed in this study outperforms current diagnostic systems with respect to sensitivity, specificity, and high-throughput detection, and thus should be useful in pathogen diagnosis in the clinical setting-
dc.description.statementOfResponsibilityopen-
dc.format.extent171~177-
dc.relation.isPartOfMOLECULAR AND CELLULAR PROBES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBacteria/genetics*-
dc.subject.MESHBacteria/isolation & purification*-
dc.subject.MESHBacteria/pathogenicity-
dc.subject.MESHBacterial Infections/diagnosis*-
dc.subject.MESHBacterial Infections/microbiology*-
dc.subject.MESHCommunicable Diseases/microbiology-
dc.subject.MESHDNA, Ribosomal Spacer/genetics-
dc.subject.MESHEnterobacter cloacae/genetics-
dc.subject.MESHEnterobacter cloacae/isolation & purification-
dc.subject.MESHEnterobacter cloacae/pathogenicity-
dc.subject.MESHEnterococcus faecium/genetics-
dc.subject.MESHEnterococcus faecium/isolation & purification-
dc.subject.MESHEnterococcus faecium/pathogenicity-
dc.subject.MESHHumans-
dc.subject.MESHOligonucleotide Array Sequence Analysis/methods*-
dc.subject.MESHReproducibility of Results-
dc.subject.MESHSensitivity and Specificity-
dc.titleHigh-throughput identification of clinically important bacterial pathogens using DNA microarray-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSeung Min Yoo-
dc.contributor.googleauthorSang Yup Lee-
dc.contributor.googleauthorKyung Hee Chang-
dc.contributor.googleauthorSo Young Yoo-
dc.contributor.googleauthorNae Choon Yoo-
dc.contributor.googleauthorKi Chang Keum-
dc.contributor.googleauthorWon Min Yoo-
dc.contributor.googleauthorJune Myung Kim-
dc.contributor.googleauthorJun Yong Choi-
dc.identifier.doi10.1016/j.mcp.2009.03.004-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00272-
dc.contributor.localIdA00953-
dc.contributor.localIdA02457-
dc.contributor.localIdA02489-
dc.contributor.localIdA03423-
dc.contributor.localIdA04191-
dc.relation.journalcodeJ02246-
dc.identifier.eissn1096-1194-
dc.identifier.pmid19374946-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0890850809000279-
dc.subject.keywordDNA Microarray-
dc.subject.keywordInfectious disease-
dc.subject.keywordBacterial pathogen-
dc.subject.keywordProbe-
dc.subject.keywordDiagnosis-
dc.contributor.alternativeNameKeum, Ki Chang-
dc.contributor.alternativeNameKim, June Myung-
dc.contributor.alternativeNameYoo, Nae Choon-
dc.contributor.alternativeNameYoo, Won Min-
dc.contributor.alternativeNameChang, Kyung Hee-
dc.contributor.alternativeNameChoi, Jun Yong-
dc.contributor.affiliatedAuthorKeum, Ki Chang-
dc.contributor.affiliatedAuthorKim, June Myung-
dc.contributor.affiliatedAuthorYoo, Nae Choon-
dc.contributor.affiliatedAuthorYoo, Won Min-
dc.contributor.affiliatedAuthorChang, Kyung Hee-
dc.contributor.affiliatedAuthorChoi, Jun Yong-
dc.citation.volume23-
dc.citation.number3-4-
dc.citation.startPage171-
dc.citation.endPage177-
dc.identifier.bibliographicCitationMOLECULAR AND CELLULAR PROBES, Vol.23(3-4) : 171-177, 2009-
dc.identifier.rimsid53745-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.