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Polymorphism of a COLIA1 gene Sp1 binding site in Korean women with pelvic organ prolapse.

 Hye Jin Ch  ;  Hyun Joo Jung  ;  Sei Kwang Kim  ;  Jong Rak Choi  ;  Nam Hoon Cho  ;  Sang Wook Bai 
 YONSEI MEDICAL JOURNAL, Vol.50(4) : 564-568, 2009 
Journal Title
Issue Date
Aged ; Asian Continental Ancestry Group/genetics ; Binding Sites/genetics ; Collagen Type I/genetics* ; Female ; Genetic Predisposition to Disease ; Humans ; Middle Aged ; Pelvic Organ Prolapse/genetics* ; Polymerase Chain Reaction ; Polymorphism, Genetic/genetics* ; Sp1 Transcription Factor/metabolism*
Polymorphism ; prolapse
PURPOSE: To evaluate the possible influence of G-->T substitution at the Sp1-binding site of the COLIA1 gene on the risk of pelvic organ prolapse (POP).

MATERIALS AND METHODS: The study group consisted of 15 women with advanced stage POP. Fifteen control subjects with uterine myomas among the postmenopausal women were matched for age and parity. DNA was obtained from peripheral blood leukocytes. The fragments of the first intron of the COLIA1 gene were amplified by real time polymerase chain reaction. The polymorphism was identified using LightCycler Technology with hybridization probes. Sequencing reactions were performed on each template using commercial primer.

RESULTS: Two groups had no significant difference in medical history, surgical, and smoking history. The homozygous peaks in two groups were noted at 57 on melting curve analysis. Sequencing reactions confirmed the G/G alleles in the 30 specimens tested. We could not find any polymorphism at the Sp1-binding site in COLIA1 gene with advanced stage POP. Statistical significance was considered to be p < .05.

CONCLUSION: The polymorphism of the Sp1-binding site in the COLIA1 gene did not contribute to the development of POP in Korea.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sei Kwang(김세광)
Bai, Sang Wook(배상욱) ORCID logo https://orcid.org/0000-0001-7724-7552
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Cho, Hye Jin(조혜진)
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
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