Cited 28 times in
Synaptic scaffolding molecule binds to and regulates vasoactive intestinal polypeptide type-1 receptor in epithelial cells.
DC Field | Value | Language |
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dc.contributor.author | 김경식 | - |
dc.contributor.author | 김경환 | - |
dc.contributor.author | 김주영 | - |
dc.contributor.author | 김호근 | - |
dc.contributor.author | 박현우 | - |
dc.contributor.author | 이민구 | - |
dc.date.accessioned | 2015-04-24T16:48:36Z | - |
dc.date.available | 2015-04-24T16:48:36Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0016-5085 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104213 | - |
dc.description.abstract | BACKGROUND & AIMS: Vasoactive intestinal polypeptide (VIP) is a principal regulator of fluid and electrolyte secretion in the gastrointestinal system. The VIP type-1 receptor (VPAC1), a class II G-protein-coupled receptor, contains a putative C-terminal PDZ-binding motif. A yeast 2-hybrid screen indicated that the C-terminus of VPAC1 bound to the PDZ domain of synaptic scaffolding molecule (S-SCAM, also known as membrane-associated guanylate kinase inverted-2 [MAGI-2]). We analyzed the association between S-SCAM and VPAC1. METHODS: The biochemical properties and physiologic significance of the interaction between VPAC1 and S-SCAM were examined in heterologous expression systems, T84 colonic epithelial cells, and human pancreas and colon tissues using an integrated molecular and physiologic approach. RESULTS: The physical interaction between VPAC1 and S-SCAM was confirmed by immunoprecipitation in HEK 293 mammalian cells and human pancreatic and colonic tissues. Immunocytochemical analysis indicated that S-SCAM recruited VPAC1 to the junctional area near the apical end of the lateral membrane in T84 cells. Several lines of evidence revealed that S-SCAM inhibits VPAC1 activation. Overexpression of S-SCAM inhibited VPAC1-mediated cAMP production and agonist-induced VPAC1 internalization in HEK 293 and HeLa cells. In addition, S-SCAM decreased the VPAC1-mediated current through the cystic fibrosis transmembrane conductance regulator in Xenopus oocytes, especially at low concentrations of VIP. Importantly, loss of S-SCAM increased VIP-induced short-circuit currents in T84 monolayers, which endogenously express VPAC1 and S-SCAM. CONCLUSIONS: S-SCAM/MAGI-2 interacts with and regulates VPAC1 intracellular localization in epithelial cells and inhibits VPAC1 agonist-induced activation and internalization. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | GASTROENTEROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Analysis of Variance | - |
dc.subject.MESH | Carrier Proteins | - |
dc.subject.MESH | Cell Communication/genetics | - |
dc.subject.MESH | Cell Communication/physiology | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Epithelial Cells/physiology | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoblotting | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Probability | - |
dc.subject.MESH | Protein Binding/genetics | - |
dc.subject.MESH | Protein Binding/physiology* | - |
dc.subject.MESH | Receptors, Vasoactive Intestinal Polypeptide, Type I/genetics | - |
dc.subject.MESH | Receptors, Vasoactive Intestinal Polypeptide, Type I/metabolism* | - |
dc.subject.MESH | Signal Transduction/genetics | - |
dc.subject.MESH | Two-Hybrid System Techniques | - |
dc.title | Synaptic scaffolding molecule binds to and regulates vasoactive intestinal polypeptide type-1 receptor in epithelial cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | HEON YUNG GEE | - |
dc.contributor.googleauthor | YOUNG WOONG KIM | - |
dc.contributor.googleauthor | MIN JAE JO | - |
dc.contributor.googleauthor | WAN NAMKUNG | - |
dc.contributor.googleauthor | JOO YOUNG KIM | - |
dc.contributor.googleauthor | HYUN WOO PARK | - |
dc.contributor.googleauthor | KYUNG SIK KIM | - |
dc.contributor.googleauthor | HOGUEN KIM | - |
dc.contributor.googleauthor | AKEMICHI BABA | - |
dc.contributor.googleauthor | JINHEE YANG | - |
dc.contributor.googleauthor | EUNJOON KIM | - |
dc.contributor.googleauthor | KYUNG HWAN KIM | - |
dc.contributor.googleauthor | MIN GOO LEE | - |
dc.identifier.doi | 10.1053/j.gastro.2009.01.065 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00942 | - |
dc.contributor.localId | A00299 | - |
dc.contributor.localId | A00311 | - |
dc.contributor.localId | A01183 | - |
dc.contributor.localId | A01743 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A03971 | - |
dc.relation.journalcode | J00917 | - |
dc.identifier.eissn | 1528-0012 | - |
dc.identifier.pmid | 19642226 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0016508509001693 | - |
dc.contributor.alternativeName | Kim, Kyung Sik | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.alternativeName | Kim, Ho Keun | - |
dc.contributor.alternativeName | Park, Hyun Woo | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Kim, Joo Young | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sik | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Ho Keun | - |
dc.contributor.affiliatedAuthor | Park, Hyun Woo | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Gee, Heon Yung | - |
dc.citation.volume | 137 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 607 | - |
dc.citation.endPage | 617.e4 | - |
dc.identifier.bibliographicCitation | GASTROENTEROLOGY, Vol.137(2) : 607-617.e4, 2009 | - |
dc.identifier.rimsid | 56116 | - |
dc.type.rims | ART | - |
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