1 216

Cited 3 times in

Development of an effective method for dendritic cell immunotherapy of mouse melanoma

Authors
 T.-H. Lee  ;  H. K. Cho  ;  Y. H. Cho  ;  M.-G. Lee 
Citation
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.70(2) : 85-92, 2009 
Journal Title
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY 
ISSN
 0300-9475 
Issue Date
2009
MeSH
Animals ; CD40 Antigens/immunology ; CD40 Antigens/metabolism ; Cell Proliferation ; Dendritic Cells/transplantation* ; Female ; Immunotherapy, Adoptive* ; Interferon-gamma/immunology ; Interferon-gamma/metabolism ; Interleukin-10/immunology ; Interleukin-10/metabolism ; Interleukin-12/immunology ; Interleukin-12/metabolism ; Interleukin-1beta/immunology ; Interleukin-1beta/metabolism ; Melanoma/therapy* ; Mice ; Mice, Inbred C57BL ; Skin Neoplasms/therapy* ; Tumor Necrosis Factor-alpha/immunology ; Tumor Necrosis Factor-alpha/metabolism
Abstract
Dendritic cell (DC) immunotherapy is a strong candidate for the treatment of incurable cancers especially malignant melanoma. Nevertheless, the proper guideline of DC immunotherapy does not exist. The absence of the guideline is also an obstacle to clinical trials of DC immunotherapy. So we conducted this study in order to develop an effective DC preparation method for immunotherapy in mouse malignant melanoma. Mouse bone marrow-derived DC were stimulated with tumour antigen alone or tumour antigen plus a cocktail (anti-CD40 antibody +TNF-alpha+ IL-1beta) for 8, 24 or 48 h and the characteristics of these DC, such as surface molecules (CD40, CD80, CD86, MHC class II, CCR7), cytokines(IL-12, IFN-gamma, and IL-10), DC-induced T cell proliferation in vitro, and the production of IFN-gamma by those cells, were evaluated. Mice with melanoma were then treated with DC stimulated with tumour antigen alone and tumour antigen plus cocktail for 8 or 48 h. The tumour size and survival rate of these mice were then evaluated. (1) Beneficial clinical effects such as a reduction of tumour size and an increased survival rate were best observed in the group treated with DC stimulated for 8 h with tumour antigen plus cocktail. (2) The single prominent characteristic of DC stimulated for 8 h with tumour antigen plus cocktail was an elevated IL-12 secretion. The cytokine IL-12 was not secreted by other DC. Consequently, proper production of IL-12 was found to be an important requirement for DC used in immunotherapy of mouse melanoma.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2009.02273.x/abstract
DOI
10.1111/j.1365-3083.2009.02273.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Min Geol(이민걸) ORCID logo https://orcid.org/0000-0001-7040-5335
Cho, Young Hun(조영훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/104101
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse