Cited 10 times in
Expression of human interferon alpha-1 with enhanced stability via the tagging system of a stabilizing peptide.
DC Field | Value | Language |
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dc.contributor.author | 김종선 | - |
dc.date.accessioned | 2015-04-24T16:29:48Z | - |
dc.date.available | 2015-04-24T16:29:48Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103622 | - |
dc.description.abstract | Human interferon alpha-1 (hIFNA1) is one of several interferon alpha subtypes that have been studied and commercialized to treat various viral diseases including hepatitis B and C as well as malignant melanoma. Protein aggregation has been problematic for every step in commercial production, from purification to the packaging and delivery of pharmaceutical proteins. In a previous study, we demonstrated that a stabilizing peptide from the C-terminal acidic tail of alpha-synuclein (ATS) could be used as an effective fusion tag to increase the stability of target proteins such as human growth hormone (hGH) and granulocyte colony-stimulating factor (G-CSF). In this study, we applied this ATS fusion system to hIFNA1 in order to protect against the aggregation of hIFNA1 by environmental stresses, since hIFNA1 aggregates elicit an undesirable immune response in humans. As expected, ATS-fused hIFNA1 (hIFNA1-ATS) protein showed enhanced stability against thermal stress, agitation stress, and repetitive freeze/thawing stress in comparison with native hIFNA1. More importantly, hIFNA1-ATS fusion protein appeared to be 1.6 times more active than hIFNA1 in a cell anti-proliferation assay. Furthermore, the solubility of hIFNA1-ATS appeared to be 1.7 times higher than that of native protein. Our results suggest that the ATS-tag system could be a useful means for protecting hIFNA1 protein from aggregation by various external stresses and could be used to increase the solubility of protein. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 140~146 | - |
dc.relation.isPartOf | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Genetic Vectors | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interferon-alpha/biosynthesis* | - |
dc.subject.MESH | Interferon-alpha/chemistry | - |
dc.subject.MESH | Interferon-alpha/isolation & purification* | - |
dc.subject.MESH | Protein Stability | - |
dc.subject.MESH | Recombinant Fusion Proteins/biosynthesis* | - |
dc.subject.MESH | Recombinant Fusion Proteins/chemistry | - |
dc.subject.MESH | Recombinant Fusion Proteins/isolation & purification* | - |
dc.subject.MESH | Transition Temperature | - |
dc.subject.MESH | alpha-Synuclein/biosynthesis | - |
dc.subject.MESH | alpha-Synuclein/chemistry | - |
dc.title | Expression of human interferon alpha-1 with enhanced stability via the tagging system of a stabilizing peptide. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Young Mok Kim | - |
dc.contributor.googleauthor | Hye Ja Lee | - |
dc.contributor.googleauthor | Jung Eun Lee | - |
dc.contributor.googleauthor | Hae Yeong Kim | - |
dc.contributor.googleauthor | Jongsun Kim | - |
dc.identifier.doi | 10.1016/j.pep.2008.09.016 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00921 | - |
dc.relation.journalcode | J02564 | - |
dc.identifier.eissn | 1096-0279 | - |
dc.identifier.pmid | 18950714 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1046592808002532 | - |
dc.subject.keyword | Interferon alpha | - |
dc.subject.keyword | Protein aggregation | - |
dc.subject.keyword | Environmental stresses | - |
dc.subject.keyword | Stability | - |
dc.subject.keyword | Protein solubility | - |
dc.contributor.alternativeName | Kim, Jong Sun | - |
dc.contributor.affiliatedAuthor | Kim, Jong Sun | - |
dc.citation.volume | 63 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 140 | - |
dc.citation.endPage | 146 | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, Vol.63(2) : 140-146, 2009 | - |
dc.identifier.rimsid | 37957 | - |
dc.type.rims | ART | - |
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