Cited 74 times in
Genetic variation in the promoter region of chitinase 3-like 1 is associated with atopy.
DC Field | Value | Language |
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dc.contributor.author | 이민구 | - |
dc.contributor.author | 이성희 | - |
dc.contributor.author | 이지현 | - |
dc.contributor.author | 김경원 | - |
dc.contributor.author | 김경환 | - |
dc.contributor.author | 김규언 | - |
dc.contributor.author | 김소원 | - |
dc.contributor.author | 손명현 | - |
dc.date.accessioned | 2015-04-24T16:28:14Z | - |
dc.date.available | 2015-04-24T16:28:14Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1073-449X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103572 | - |
dc.description.abstract | RATIONALE: Atopy or atopic syndrome is an allergic hypersensitivity subject to hereditary influences. Aberrant expression of chitinase 3-like 1 (CHI3L1), also known as YKL-40 or HC gp-39, is involved in the pathogenesis of inflammatory and allergic diseases. OBJECTIVES: The genetic contribution of CHI3L1 gene to atopic susceptibility was investigated using an integrated population genetic and molecular analysis. METHODS: Genetic variations in CHI3L1 were identified and genotyped in 295 unrelated patients with atopy and 180 control subjects. Serum YKL-40 and IgE levels were analyzed according to genotype. The effects of a promoter polymorphism (g.-247C/T) on promoter activity were examined in reporter and protein binding assays. MEASUREMENTS AND MAIN RESULTS: In the case-control association analysis, the g.-247C/T polymorphism at the promoter region (rs10399805; P = 0.0062) and the IVS7+82C/T polymorphism at intron 7 (rs2275353; P = 0.0056) of CHI3L1 showed a significant association with atopy. Subjects with the g.-247T risk allele had significantly higher serum YKL-40 (P < 0.0001) and IgE (P = 0.012) levels. An in vitro promoter assay using THP-1 human monocyte cells revealed that the C to T conversion at g.-247 induced a more than twofold increase of reporter gene expression. Moreover, the g.-247T allele showed an increased affinity for CCAAT enhancer-binding protein, a well known transcriptional activator, by electrophoretic mobility shift assay. Accordingly, subjects with the g.-247TT genotype showed a 2.5-fold increase in CHI3L1 mRNA expression in peripheral blood cells compared with those with the g.-247CC genotype. CONCLUSIONS: These results strongly suggest that the g.-247C/T polymorphism in the CHI3L1 promoter region is associated with the risk of atopy | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 449~456 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipokines | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Proteins | - |
dc.subject.MESH | Case-Control Studies | - |
dc.subject.MESH | Child | - |
dc.subject.MESH | Chitinase-3-Like Protein 1 | - |
dc.subject.MESH | Electrophoretic Mobility Shift Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Genotype | - |
dc.subject.MESH | Glycoproteins/blood | - |
dc.subject.MESH | Glycoproteins/genetics* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypersensitivity, Immediate/genetics* | - |
dc.subject.MESH | Immunoglobulin E/blood | - |
dc.subject.MESH | Korea | - |
dc.subject.MESH | Lectins | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Polymorphism, Single Nucleotide* | - |
dc.subject.MESH | Promoter Regions, Genetic* | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Risk Factors | - |
dc.title | Genetic variation in the promoter region of chitinase 3-like 1 is associated with atopy. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아과학) | - |
dc.contributor.googleauthor | Myung Hyun Sohn | - |
dc.contributor.googleauthor | Ji Hyun Lee | - |
dc.contributor.googleauthor | Kyung Won Kim | - |
dc.contributor.googleauthor | So Won Kim | - |
dc.contributor.googleauthor | Sung Hee Lee | - |
dc.contributor.googleauthor | Kyu-Earn Kim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Chun Geun Lee | - |
dc.contributor.googleauthor | Jack A. Elias | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.identifier.doi | 10.1164/rccm.200809-1422OC | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03217 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A02876 | - |
dc.contributor.localId | A00303 | - |
dc.contributor.localId | A00311 | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A00622 | - |
dc.contributor.localId | A01967 | - |
dc.relation.journalcode | J00112 | - |
dc.identifier.eissn | 1535-4970 | - |
dc.identifier.pmid | 19106306 | - |
dc.identifier.url | http://www.atsjournals.org/doi/abs/10.1164/rccm.200809-1422OC?journalCode=ajrccm& | - |
dc.subject.keyword | chitinase-like proteins | - |
dc.subject.keyword | genetic association | - |
dc.subject.keyword | YKL-40 | - |
dc.subject.keyword | hypersensitivity | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.alternativeName | Lee, Sung Hee | - |
dc.contributor.alternativeName | Lee, Ji Hyun | - |
dc.contributor.alternativeName | Kim, Kyung Won | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Kim, So Won | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Ji Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Lee, Sung Hee | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Won | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Kim, So Won | - |
dc.contributor.affiliatedAuthor | Son, Myung Hyun | - |
dc.citation.volume | 179 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 449 | - |
dc.citation.endPage | 456 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Vol.179(6) : 449-456, 2009 | - |
dc.identifier.rimsid | 37931 | - |
dc.type.rims | ART | - |
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