1 206

Cited 58 times in

Genetic variation in the promoter region of chitinase 3-like 1 is associated with atopy.

DC FieldValueLanguage
dc.contributor.author이민구-
dc.contributor.author이성희-
dc.contributor.author이지현-
dc.contributor.author김경원-
dc.contributor.author김경환-
dc.contributor.author김규언-
dc.contributor.author김소원-
dc.contributor.author손명현-
dc.date.accessioned2015-04-24T16:28:14Z-
dc.date.available2015-04-24T16:28:14Z-
dc.date.issued2009-
dc.identifier.issn1073-449X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103572-
dc.description.abstractRATIONALE: Atopy or atopic syndrome is an allergic hypersensitivity subject to hereditary influences. Aberrant expression of chitinase 3-like 1 (CHI3L1), also known as YKL-40 or HC gp-39, is involved in the pathogenesis of inflammatory and allergic diseases. OBJECTIVES: The genetic contribution of CHI3L1 gene to atopic susceptibility was investigated using an integrated population genetic and molecular analysis. METHODS: Genetic variations in CHI3L1 were identified and genotyped in 295 unrelated patients with atopy and 180 control subjects. Serum YKL-40 and IgE levels were analyzed according to genotype. The effects of a promoter polymorphism (g.-247C/T) on promoter activity were examined in reporter and protein binding assays. MEASUREMENTS AND MAIN RESULTS: In the case-control association analysis, the g.-247C/T polymorphism at the promoter region (rs10399805; P = 0.0062) and the IVS7+82C/T polymorphism at intron 7 (rs2275353; P = 0.0056) of CHI3L1 showed a significant association with atopy. Subjects with the g.-247T risk allele had significantly higher serum YKL-40 (P < 0.0001) and IgE (P = 0.012) levels. An in vitro promoter assay using THP-1 human monocyte cells revealed that the C to T conversion at g.-247 induced a more than twofold increase of reporter gene expression. Moreover, the g.-247T allele showed an increased affinity for CCAAT enhancer-binding protein, a well known transcriptional activator, by electrophoretic mobility shift assay. Accordingly, subjects with the g.-247TT genotype showed a 2.5-fold increase in CHI3L1 mRNA expression in peripheral blood cells compared with those with the g.-247CC genotype. CONCLUSIONS: These results strongly suggest that the g.-247C/T polymorphism in the CHI3L1 promoter region is associated with the risk of atopy-
dc.description.statementOfResponsibilityopen-
dc.format.extent449~456-
dc.relation.isPartOfAmerican Journal of Respiratory and Critical Care Medicine-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleGenetic variation in the promoter region of chitinase 3-like 1 is associated with atopy.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pediatrics (소아과학)-
dc.contributor.googleauthorMyung Hyun Sohn-
dc.contributor.googleauthorJi Hyun Lee-
dc.contributor.googleauthorKyung Won Kim-
dc.contributor.googleauthorSo Won Kim-
dc.contributor.googleauthorSung Hee Lee-
dc.contributor.googleauthorKyu-Earn Kim-
dc.contributor.googleauthorKyung Hwan Kim-
dc.contributor.googleauthorChun Geun Lee-
dc.contributor.googleauthorJack A. Elias-
dc.contributor.googleauthorMin Goo Lee-
dc.identifier.doi10.1164/rccm.200809-1422OC-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03217-
dc.contributor.localIdA02781-
dc.contributor.localIdA02876-
dc.contributor.localIdA00303-
dc.contributor.localIdA00311-
dc.contributor.localIdA00327-
dc.contributor.localIdA00622-
dc.contributor.localIdA01967-
dc.relation.journalcodeJ00112-
dc.identifier.urlhttp://www.atsjournals.org/doi/abs/10.1164/rccm.200809-1422OC?journalCode=ajrccm&-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.alternativeNameLee, Sung Hee-
dc.contributor.alternativeNameLee, Ji Hyun-
dc.contributor.alternativeNameKim, Kyung Won-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.alternativeNameKim, Kyu Earn-
dc.contributor.alternativeNameKim, So Won-
dc.contributor.alternativeNameSon, Myung Hyun-
dc.contributor.affiliatedAuthorLee, Ji Hyun-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.contributor.affiliatedAuthorLee, Sung Hee-
dc.contributor.affiliatedAuthorKim, Kyung Won-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.contributor.affiliatedAuthorKim, Kyu Earn-
dc.contributor.affiliatedAuthorKim, So Won-
dc.contributor.affiliatedAuthorSon, Myung Hyun-
dc.citation.volume179-
dc.citation.number6-
dc.citation.startPage449-
dc.citation.endPage456-
dc.identifier.bibliographicCitationAmerican Journal of Respiratory and Critical Care Medicine, Vol.179(6) : 449-456, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.