1 526

Cited 46 times in

Influence of microgroove dimension on cell behavior of human gingival fibroblasts cultured on titanium substrata

DC Field Value Language
dc.contributor.author이근우-
dc.contributor.author정원윤-
dc.date.accessioned2015-04-24T16:25:40Z-
dc.date.available2015-04-24T16:25:40Z-
dc.date.issued2009-
dc.identifier.issn0905-7161-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103493-
dc.description.abstractOBJECTIVE: The purpose of this study was to determine the dimension of surface microgrooves on titanium (Ti) substrata that shows the greatest positive influence on characterizing specific cell behavior of cultured human gingival fibroblasts. MATERIAL AND METHODS: Commercially pure Ti disks with surface microgrooves of monotonous 3.5 mm in depth and respective 15, 30, and 60 microm in width were fabricated using photolithography and used as the culture substrata in the three experimental groups in this study (TiD15, TiD30, and TiD60 groups), whereas the smooth Ti disk was used as the control substrata (smooth Ti group). Human gingival fibroblasts were cultured on the four groups of Ti substrata on successive timelines. Cell behaviors, such as adhesion, morphology,viability and proliferation, and gene expression were analyzed and compared between all groups using crystal violet stain, scanning electron microscopy (SEM), XTT assay, and reverse transcriptase-polymerase chain reaction, respectively. RESULTS: SEM demonstrated that cells were able to readily descend into the microgrooves of TiD30 at the early phase of culture. Cells on the ridge edges or in groove corners were spindle shaped with abundant filopodia formation toward the acid-etched surface inside the microgrooves, thus mimicked the cell shape in three-dimensional (3D) nanoenvironment. TiD15 significantly increased the cell viability and proliferation compared with the smooth Ti substrata after 72 h of culture. Up-regulation of fibronectin(FN) and alpha5 integrin genes was noted in cells cultured on TiD15 and TiD30. Gene expression pattern specific to the cells in 3D-matrix culture, such as down-regulation of alpha-smooth muscle actin gene along with up-regulation of FN and p21 genes, was pronounced in cells cultured on TiD30. CONCLUSION: This study indicates that surface microgrooves of both 15 and 30 microm in widt hand a monotonous 3.5 microm in depth on Ti substrata increase various cell behaviors of cultured human gingival fibroblasts.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfCLINICAL ORAL IMPLANTS RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHActins/biosynthesis-
dc.subject.MESHAlkaline Phosphatase/biosynthesis-
dc.subject.MESHAlkaline Phosphatase/genetics-
dc.subject.MESHCell Adhesion-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCell Shape-
dc.subject.MESHCell Survival-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p21/biosynthesis-
dc.subject.MESHDental Etching*-
dc.subject.MESHFibroblasts/cytology-
dc.subject.MESHFibroblasts/physiology*-
dc.subject.MESHFibronectins/biosynthesis-
dc.subject.MESHGene Expression-
dc.subject.MESHGingiva/cytology*-
dc.subject.MESHGingiva/physiology*-
dc.subject.MESHHumans-
dc.subject.MESHIntegrin alpha5/biosynthesis-
dc.subject.MESHMicroscopy, Electron, Scanning-
dc.subject.MESHSurface Properties-
dc.subject.MESHTitanium*-
dc.titleInfluence of microgroove dimension on cell behavior of human gingival fibroblasts cultured on titanium substrata-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Prosthodontics (보철과학)-
dc.contributor.googleauthorSuk-Won Lee-
dc.contributor.googleauthorSu-Yeon Kim-
dc.contributor.googleauthorIn-Chul Rhyu-
dc.contributor.googleauthorWon-Yoon Chung-
dc.contributor.googleauthorRichard Leesungbok-
dc.contributor.googleauthorKeun-Woo Lee-
dc.identifier.doi10.1111/j.1600-0501.2008.01597.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02688-
dc.contributor.localIdA02852-
dc.contributor.localIdA03676-
dc.relation.journalcodeJ00600-
dc.identifier.eissn1600-0501-
dc.identifier.pmid19133333-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1600-0501.2008.01597.x/abstract-
dc.subject.keywordfibroblast-
dc.subject.keywordgene expression-
dc.subject.keywordmicrogroove-
dc.subject.keywordproliferation-
dc.subject.keywordtitanium substrata-
dc.contributor.alternativeNameLee, Keun Woo-
dc.contributor.alternativeNameChung, Won Yoon-
dc.contributor.affiliatedAuthorLee, Keun Woo-
dc.contributor.affiliatedAuthorChung, Won Yoon-
dc.citation.volume20-
dc.citation.number1-
dc.citation.startPage56-
dc.citation.endPage66-
dc.identifier.bibliographicCitationCLINICAL ORAL IMPLANTS RESEARCH, Vol.20(1) : 56-66, 2009-
dc.identifier.rimsid36039-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Prosthodontics (보철과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.