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Identification of the amino acid sequence motif of alpha-synuclein responsible for macrophage activation

DC FieldValueLanguage
dc.contributor.author김종선-
dc.contributor.author박상면-
dc.contributor.author안근재-
dc.date.accessioned2015-04-24T16:25:20Z-
dc.date.available2015-04-24T16:25:20Z-
dc.date.issued2009-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/103483-
dc.description.abstractAlpha-synuclein (Syn) is implicated in the pathogenesis of PD and related neurodegenerative disorders. Recent studies have also shown that alpha-synuclein can activate microglia and enhance dopaminergic neurodegeneration. The mechanisms of microglia activation by alpha-synuclein, however, are not well understood. In this study, we found that not only alpha-synuclein but also beta- and gamma-synucleins activated macrophages (RAW 264.7) in vitro. Macrophages treated with synuclein proteins secreted TNF-alpha in a dose-dependent manner. Synuclein family proteins also increased mRNA transcription of COX-2 and iNOS. Two alpha-synuclein deletion mutants, SynDeltaNAC and Syn61-140, activated macrophages, while deletion mutants Syn1-60 and Syn96-140 did not significantly activate them. Finally, we demonstrated that macrophage activation by alpha-synuclein was accompanied by phosphorylation of ERK. These results suggest that synuclein family proteins can activate macrophages, and that macrophage activation needs both the N-terminal and C-terminal domains of alpha-synuclein, but not the central NAC region.-
dc.description.statementOfResponsibilityopen-
dc.format.extent39~43-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Motifs/genetics-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line-
dc.subject.MESHDNA Mutational Analysis-
dc.subject.MESHHumans-
dc.subject.MESHMacrophage Activation*/genetics-
dc.subject.MESHMacrophages/drug effects-
dc.subject.MESHMacrophages/immunology*-
dc.subject.MESHMice-
dc.subject.MESHRecombinant Proteins/genetics-
dc.subject.MESHRecombinant Proteins/metabolism-
dc.subject.MESHSequence Analysis, Protein-
dc.subject.MESHSequence Deletion-
dc.subject.MESHalpha-Synuclein/genetics-
dc.subject.MESHalpha-Synuclein/pharmacology-
dc.subject.MESHalpha-Synuclein/physiology*-
dc.titleIdentification of the amino acid sequence motif of alpha-synuclein responsible for macrophage activation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Dermatology (피부과학)-
dc.contributor.googleauthorSaet-byul Lee-
dc.contributor.googleauthorSang Myun Park-
dc.contributor.googleauthorKeun Jae Ahn-
dc.contributor.googleauthorKwang Chul Chung-
dc.contributor.googleauthorSeung R. Paik-
dc.contributor.googleauthorJongsun Kim-
dc.identifier.doi10.1016/j.bbrc.2009.02.002-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00921-
dc.contributor.localIdA01484-
dc.contributor.localIdA02222-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid19351591-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X09002460-
dc.subject.keywordSynuclein-
dc.subject.keywordTNF-α-
dc.subject.keywordMacrophage-
dc.subject.keywordInflammation-
dc.subject.keywordParkinson’s disease-
dc.contributor.alternativeNameKim, Jong Sun-
dc.contributor.alternativeNamePark, Sang Myun-
dc.contributor.alternativeNameAhn, Keun Jae-
dc.contributor.affiliatedAuthorKim, Jong Sun-
dc.contributor.affiliatedAuthorPark, Sang Myun-
dc.contributor.affiliatedAuthorAhn, Keun Jae-
dc.citation.volume381-
dc.citation.number1-
dc.citation.startPage39-
dc.citation.endPage43-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.381(1) : 39-43, 2009-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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