Cited 9 times in
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one inhibits neurite outgrowth and causes neurite retraction in PC12 cells independently of soluble guanylyl cyclase
DC Field | Value | Language |
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dc.contributor.author | 김소영 | - |
dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 이승일 | - |
dc.contributor.author | 이한길 | - |
dc.contributor.author | 김두식 | - |
dc.date.accessioned | 2015-04-24T16:23:23Z | - |
dc.date.available | 2015-04-24T16:23:23Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0360-4012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103424 | - |
dc.description.abstract | The effect of the potent soluble guanylyl cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) on neurite outgrowth and retraction was investigated in PC12 cells and SH-SY5Y human neuroblastoma cells. ODQ inhibited neurite outgrowth and triggered neurite retraction in the cells stimulated with nerve growth factor (NGF), staurosporine, or Y-27632. The nitric oxide (NO) scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (PTIO) had little effect on neurite outgrowth induced by Y-27632 or staurosporine. In the presence of ODQ, treatment of the cells with the cell-permeable cGMP analogue 8-bromo-cGMP failed to retrigger Y-27632- and staurosporine-induced neurite outgrowth. Furthermore, the depletion of sGC by RNA interference failed to prevent Y-27632- and staurosporine-induced neurite outgrowth. These results indicate that the NO/sGC/cGMP signaling cascade is not critically involved in ODQ-induced neurite remodeling. The MEK inhibitor PD98059 did not inhibit neurite outgrowth, and Y-27632 and staurosporine did not induce ERK phosphorylation, suggesting that the inhibitory effect of ODQ on neurite outgrowth is independent of the ERK signaling pathway. In contrast, pretreatment with dithionite or a hemin-glutathione mixture reversed the inhibitory effect of ODQ on Y-27632- and staurosporine-induced neurite outgrowth, indicating that ODQ might act on an intracellular redox-sensitive molecule. We conclude that ODQ inhibits Y-27632- and staurosporine-induced neurite outgrowth and triggers neurite retraction in an sGC-independent manner in neuronal cells and suggest that oxidation of unidentified redox-sensitive protein could be responsible for these effects | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 269~277 | - |
dc.relation.isPartOf | JOURNAL OF NEUROSCIENCE RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology* | - |
dc.subject.MESH | Guanylate Cyclase/antagonists & inhibitors | - |
dc.subject.MESH | Guanylate Cyclase/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Nerve Growth Factor/pharmacology | - |
dc.subject.MESH | Neurites/drug effects* | - |
dc.subject.MESH | Oxadiazoles/pharmacology* | - |
dc.subject.MESH | PC12 Cells/cytology | - |
dc.subject.MESH | Quinoxalines/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Receptors, Cytoplasmic and Nuclear/antagonists & inhibitors | - |
dc.subject.MESH | Receptors, Cytoplasmic and Nuclear/metabolism* | - |
dc.subject.MESH | Soluble Guanylyl Cyclase | - |
dc.title | 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one inhibits neurite outgrowth and causes neurite retraction in PC12 cells independently of soluble guanylyl cyclase | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Han Gil Lee | - |
dc.contributor.googleauthor | So Young Kim | - |
dc.contributor.googleauthor | Du Sik Kim | - |
dc.contributor.googleauthor | Su Ryeon Seo | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Patrick De Smet | - |
dc.contributor.googleauthor | Jeong Taeg Seo | - |
dc.identifier.doi | 10.1002/jnr.21838 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02924 | - |
dc.contributor.localId | A03273 | - |
dc.contributor.localId | A00417 | - |
dc.contributor.localId | A00620 | - |
dc.relation.journalcode | J01634 | - |
dc.identifier.eissn | 1097-4547 | - |
dc.identifier.pmid | 18711750 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jnr.21838/abstract | - |
dc.subject.keyword | Y‐27632 | - |
dc.subject.keyword | staurosporine | - |
dc.subject.keyword | redox‐sensitive proteins | - |
dc.subject.keyword | Rho | - |
dc.contributor.alternativeName | Kim, So Young | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Lee, Syng Ill | - |
dc.contributor.alternativeName | Lee, Han Gil | - |
dc.contributor.alternativeName | Kim, Du Sik | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Syng Ill | - |
dc.contributor.affiliatedAuthor | Lee, Han Gil | - |
dc.contributor.affiliatedAuthor | Kim, Du Sik | - |
dc.contributor.affiliatedAuthor | Kim, So Young | - |
dc.citation.volume | 87 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 269 | - |
dc.citation.endPage | 277 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROSCIENCE RESEARCH, Vol.87(1) : 269-277, 2009 | - |
dc.identifier.rimsid | 37331 | - |
dc.type.rims | ART | - |
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