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Outbreak of meropenem-resistant Serratia marcescens comediated by chromosomal AmpC beta-lactamase overproduction and outer membrane protein loss

 Borum Suh  ;  Il Kwon Bae  ;  Juwon Kim  ;  Seok Hoon Jeong  ;  Dongeun Yong  ;  Kyungwon Lee 
 Antimicrobial Agents and Chemotherapy, Vol.54(12) : 5057-5061, 2010 
Journal Title
 Antimicrobial Agents and Chemotherapy 
Issue Date
Adolescent ; Adult ; Aged ; Anti-Bacterial Agents/pharmacology* ; Anti-Bacterial Agents/therapeutic use ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism* ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism* ; Blotting, Southern ; Drug Resistance, Bacterial*/genetics ; Female ; Humans ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Molecular Sequence Data ; Polymerase Chain Reaction ; Porins/genetics ; Porins/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Serratia Infections/drug therapy ; Serratia Infections/microbiology* ; Serratia marcescens/drug effects* ; Serratia marcescens/genetics ; Thienamycins*/pharmacology ; Young Adult ; beta-Lactamases/genetics ; beta-Lactamases/metabolism*
resistant (MIC range, 16 to 32 μg/ml) S. marcescens isolates were recovered from nine patients in a tertiary hospital in Seoul, South Korea, from June to November 2005. All the isolates shared identical or similar (>85% similarity) SpeI macrorestriction patterns, indicating clonal spread. PCR experiments did not detect any carbapenemase in those isolates. They carried the bla(CTX-M-22) gene located on a 150-kbp plasmid of the incompatibility group L/M; however, the addition of clavulanic acid exhibited few effects on meropenem MICs. Although meropenem MICs were reduced 4- to 16-fold with the addition of boronic acid, no plasmid-borne AmpC β-lactamase gene was detected in PCR experiments. Real-time quantitative PCR experiments showed that expression levels of the chromosomal ampC gene in those isolates were 87.06 to 155.76 times higher than that of the reference strain ATCC 8100. SDS-PAGE showed a lack of the 42-kDa outer membrane protein (OmpF). In combination with the overproduction of the chromosomal AmpC enzyme, the loss of OmpF may have played a role in the acquisition of meropenem resistance in our isolates.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Won(김주원)
Bae, Il Kwon(배일권)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
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