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Human embryonic stem cells (hESCs) cultured under distinctive feeder-free culture conditions display global gene expression patterns similar to hESCs from feeder-dependent culture conditions

Authors
 Tae-Min Yoon  ;  Bomi Chang  ;  Hyeung-Taek Kim  ;  Joo-Hyun Jee  ;  Dong-Wook Kim  ;  Dong-Youn Hwang 
Citation
 STEM CELL REVIEWS AND REPORTS, Vol.6(3) : 425-437, 2010 
Journal Title
STEM CELL REVIEWS AND REPORTS
ISSN
 2629-3269 
Issue Date
2010
MeSH
Biomarkers/metabolism ; CellCultureTechniques/methods ; Cell Differentiation/genetics ; Cell Proliferation ; Cells,Cultured ; Coculture Techniques/methods ; EmbryonicStemCells/cytology* ; EmbryonicStemCells/metabolism* ; EmbryonicStemCells/physiology ; GeneExpressionProfiling ; GeneExpressionRegulation* ; Genome,Human ; Humans
Keywords
Feeder-free culture ; Feeder-dependent culture ; Human embryonic stem cells ; Gene-expression profile
Abstract
Human embryonic stem cell (hESC)-based assay systems and genetically modified hESCs are very useful tools for screening drugs that regulate stemness and differentiation and for studying the molecular mechanisms involved in hESC fate determination. For these types of studies, feeder cell-dependent cultures of hESCs are often problematic because the physiology of the feeder cells is perturbed by the drug treatments or genetic modifications, which potentially obscures research outcomes. In this study, we evaluated three commonly used feeder-free culture conditions to determine whether they supported the undifferentiated growth of hESCs and to determine whether the hESCs grown in these conditions displayed gene expression patterns that were similar to the expression patterns of feeder cell-dependent hESCs. Our results demonstrate that hESCs grown in the three feeder-free conditions expressed undifferentiation marker genes as strongly as hESCs that were grown in the feeder-dependent cultures. Furthermore, genome-wide gene expression profiles indicated that the gene expression patterns of hESCs that were grown under feeder-free or feeder-dependent culture conditions were highly similar. These results indicate that the feeder-free culture conditions support the undifferentiated growth of hESCs as effectively as the feeder-dependent culture conditions. Therefore, feeder-free culture conditions are potentially suitable for drug screening and for the genetic manipulation of hESCs in basic research.
Full Text
http://link.springer.com/article/10.1007%2Fs12015-010-9158-x
DOI
10.1007/s12015-010-9158-x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103053
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