치아보관방법에 따른 우치 법랑질의 표면변화

Abstract

Objectives. The aim of this study was to assess the influence of storage methods on the enamel specimen. Methods. Each enamel specimen (3 mm in diameter) from bovine anterior teeth was stored in 5 mL of 6 kinds of media which were saline, distilled water, thymol solution (0.1%), Hank`s balanced salts solution (HBSS), ethanol (70%) and frozen at -20℃ for 25 and 125 days. In order to evaluate effects of each storage method, surface micro hardness (VHN) of enamel surface was measured before and after storage of specimens. Also, confocal laser scanning microscope (CLSM) and polarized light microscope (PLM) were used to observe the sagittal section of the enamel specimen. Results. After storage for 25 days in the saline, distilled water, 0.1% thymol solution and HBSS media, the micro hardness of the enamel surface have decreased significantly (p＜0.01). In contrast, storing teeth in 70% ethanol and freezing at -20℃ did not have significant difference in the micro hardness before and after storage. After storage for 125 days, only freezing at -20℃ showed no storage effect to the enamel surface. Conclusions. It was concluded that 70% ethanol and freezing at -20℃ with no surface contact of water could be proper methods for storage of teeth in micro hardness study. So, we recommend that extracted teeth and tooth specimens should be shortly stored in the 70% ethanol for disinfection process, and then be stored with freezing temperature of -20℃ with no surface contact of water