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Expression and possible role of non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) in the human endometrium and endome

Authors
 Seok Kyo Seo  ;  Anna Nam  ;  Young Eun Jeon  ;  SiHyun Cho  ;  Young Sik Choi  ;  Byung Seok Lee 
Citation
 HUMAN REPRODUCTION, Vol.25(12) : 3043-3049, 2010 
Journal Title
 HUMAN REPRODUCTION 
ISSN
 0268-1161 
Issue Date
2010
MeSH
Adult ; Apoptosis/drug effects ; Celecoxib ; Cyclooxygenase 2 Inhibitors/pharmacology ; Endometriosis/physiopathology* ; Endometrium/metabolism* ; Endometrium/physiology ; Female ; Growth Differentiation Factor 15/biosynthesis* ; Growth Differentiation Factor 15/physiology ; Humans ; Menstrual Cycle ; Middle Aged ; Pyrazoles/pharmacology ; RNA, Messenger/metabolism ; Stromal Cells/metabolism ; Sulfonamides/pharmacology
Keywords
NAG-1 ; endometrium ; endometriosis ; apoptosis
Abstract
BACKGROUND: Non-steroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1) is involved in cellular processes such as inflammation, apoptosis and tumorigenesis. However, little is known about the expression and function of NAG-1 in the endometrium. This study aimed to evaluate the expression of NAG-1 in the endometrium and in the absence or presence of endometriosis and to investigate the effect of celecoxib, a selective cyclooxygenase (COX)-2 inhibitor, on NAG-1 mRNA levels and apoptosis in human endometrial stromal cells (HESCs). METHODS: Eutopic endometrial samples were obtained during surgery from 40 patients with, and 40 patients without, endometriosis. Real-time PCR was used to quantify NAG-1 mRNA levels and immunohistochemistry was used to localize NAG-1 protein in the endometrium. To investigate the effects of celecoxib, HESCs were isolated and cultured with different concentrations of celecoxib or with 100 µM celecoxib at different times. Apoptosis was assessed by flow cytometry. RESULTS: NAG-1 mRNA levels and immunoreactivity showed cyclical changes through the menstrual cycle, increasing during the late secretory and menstrual phases. NAG-1 mRNA and protein levels were significantly lower in patients with endometriosis, compared with the control group. Celecoxib induced NAG-1 mRNA levels and apoptosis in cultured HESCs, with the effects dependent on drug concentrations and duration of treatment. Celecoxib treatment had no effect on prostaglandin E(2) levels in the culture supernatants. CONCLUSIONS: NAG-1 may be important in maintaining homeostasis in the normal endometrium and alterations in NAG-1 expression may be associated with the establishment of endometriosis. NAG-1 might be a therapeutic target for endometriosis
Files in This Item:
T201004875.pdf Download
DOI
10.1093/humrep/deq277
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
Lee, Byung Seok(이병석) ORCID logo https://orcid.org/0000-0001-6001-2079
Jeon, Young Eun(전영은)
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
Choi, Young Sik(최영식) ORCID logo https://orcid.org/0000-0002-1157-4822
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102748
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