Cited 14 times in
Controlled nonviral gene delivery and expression using stable neural stem cell line transfected with a hypoxia-inducible gene expression system
DC Field | Value | Language |
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dc.contributor.author | 김효진 | - |
dc.contributor.author | 안성수 | - |
dc.contributor.author | 오진수 | - |
dc.contributor.author | 윤도흠 | - |
dc.contributor.author | 하윤 | - |
dc.contributor.author | 김긍년 | - |
dc.date.accessioned | 2015-04-23T17:30:23Z | - |
dc.date.available | 2015-04-23T17:30:23Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1099-498X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102595 | - |
dc.description.abstract | BACKGROUND: Nonviral ex vivo local gene therapy systems consisting of regulated gene expression vectors and cellular delivery platforms represent a novel strategy for tissue repair and regeneration. We introduced a hypoxia-regulated plasmid-based system into mouse neural stem cells (NSCs) as an efficient gene expression and delivery platform for rapid, robust and persistent hypoxic/ischemic-regulated gene expression in the spinal cord. METHODS: A synthetic hypoxia-responsive erythropoietin (Epo) enhancer, the SV40 minimal promoter and the luciferase (Luc) reporter gene were incorporated in a DsRed-expressing double-promoter plasmid for cell lipofection and Zeocin-selection to establish a hypoxia-regulated stable NSC line (NSC-Epo-SV-Luc). A nonhypoxia-regulated stable NSC line (NSC-SV-Luc) was also established as a control. RESULTS: Under the transcriptional regulation of the Epo enhancer, in vitro luciferase expression in NSC-Epo-SV-Luc, but not in NSC-SV-Luc, was sensitively augmented according to the strength and duration of the hypoxic stimulus and was quickly down-regulated to a low basal level after reoxygenation of the hypoxic cells. Furthermore, deoxygenation of the reoxygenated cells clearly enhanced the luciferase activity again. After transplantation into a rat spinal cord injury (SCI) model, only NSC-Epo-SV-Luc showed ischemic injury-specific luciferase expression Notably, the engineered NSC lines kept the neural differentiation potential and retained the hypoxia-regulated luciferase expression after differentiation. CONCLUSIONS: We propose that NSCs engineered with the Epo-SV-therapeutic gene will be valuable for developing a controllable stem cell-mediated nonviral gene therapy for SCI or other central nervous system diseases accompanied with chronic or episodic hypoxic/ischemic stresses | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 990~1001 | - |
dc.relation.isPartOf | JOURNAL OF GENE MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Erythropoietin | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Gene Transfer Techniques | - |
dc.subject.MESH | Genetic Therapy/methods* | - |
dc.subject.MESH | Genetic Vectors | - |
dc.subject.MESH | Hypoxia* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neural Stem Cells/metabolism | - |
dc.subject.MESH | Neural Stem Cells/transplantation* | - |
dc.subject.MESH | Simian virus 40/genetics | - |
dc.subject.MESH | Transfection/methods* | - |
dc.title | Controlled nonviral gene delivery and expression using stable neural stem cell line transfected with a hypoxia-inducible gene expression system | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | Meng-Lu Liu | - |
dc.contributor.googleauthor | Jin Soo Oh | - |
dc.contributor.googleauthor | Sung Su An | - |
dc.contributor.googleauthor | William A. Pennant | - |
dc.contributor.googleauthor | Hyo Jin Kim | - |
dc.contributor.googleauthor | So-Jung Gwak | - |
dc.contributor.googleauthor | Do Heum Yoon | - |
dc.contributor.googleauthor | Keung Nyun Kim | - |
dc.contributor.googleauthor | Minhyung Lee | - |
dc.contributor.googleauthor | Yoon Ha | - |
dc.identifier.doi | 10.1002/jgm.1527 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01206 | - |
dc.contributor.localId | A02235 | - |
dc.contributor.localId | A02401 | - |
dc.contributor.localId | A02546 | - |
dc.contributor.localId | A04255 | - |
dc.contributor.localId | A00331 | - |
dc.relation.journalcode | J01419 | - |
dc.identifier.eissn | 1521-2254 | - |
dc.identifier.pmid | 21157823 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jgm.1527/abstract | - |
dc.contributor.alternativeName | Kim, Hyo Jin | - |
dc.contributor.alternativeName | An, Sung Su | - |
dc.contributor.alternativeName | Oh, Jin Soo | - |
dc.contributor.alternativeName | Yoon, Do Heum | - |
dc.contributor.alternativeName | Ha, Yoon | - |
dc.contributor.alternativeName | Kim, Keung Nyun | - |
dc.contributor.affiliatedAuthor | Kim, Hyo Jin | - |
dc.contributor.affiliatedAuthor | An, Sung Su | - |
dc.contributor.affiliatedAuthor | Oh, Jin Soo | - |
dc.contributor.affiliatedAuthor | Yoon, Do Heum | - |
dc.contributor.affiliatedAuthor | Ha, Yoon | - |
dc.contributor.affiliatedAuthor | Kim, Keung Nyun | - |
dc.citation.volume | 12 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 990 | - |
dc.citation.endPage | 1001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENE MEDICINE, Vol.12(12) : 990-1001, 2010 | - |
dc.identifier.rimsid | 55317 | - |
dc.type.rims | ART | - |
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