Cited 15 times in
Manufacturing of insulin-secreting spheroids with the RIN-5F cell line using a shaking culture method
DC Field | Value | Language |
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dc.contributor.author | 김명수 | - |
dc.contributor.author | 김유선 | - |
dc.contributor.author | 김준예 | - |
dc.contributor.author | 정진호 | - |
dc.contributor.author | 조유리 | - |
dc.contributor.author | 주동진 | - |
dc.contributor.author | 주만기 | - |
dc.contributor.author | 허규하 | - |
dc.date.accessioned | 2015-04-23T17:29:26Z | - |
dc.date.available | 2015-04-23T17:29:26Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102564 | - |
dc.description.abstract | BACKGROUND: There have been many efforts to find methods to increase insulin production by islets or modified cells. Commercially available established cell lines can be a good source of artificial islets. We manufactured sphere-shaped cell clusters composed of insulin-secreting cells from the commercially available RIN-5F cell line. METHODS: To generate artificial islets with insulin-secretion functions, we used the RIN-5F cell line. When cells cultured in RPMI-1640 medium containing 10% fetal bovine serum reached near confluency, they were trypsinized for suspension culture at high density, using a horizontal shaker. The cells were maintained for 5 days under 5% CO(2) with humidification. Next, the media from the RIN cell spheroid culture was collected over 5 consecutive days to test for insulin secretion. RESULTS: Spheroids of artificial islets exhibited an oval shape with an approximate size of 94.13 ± 20.41 μm on day 5 during the shaking culture. Abnormal outgrowth of spheroids was not observed. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive cells were not detected among the overall spheroids, including the core position. Insulin secretion, measured by enzyme-linked immunosorbent assay, was well maintained in the culture media over 5 days after spheroid formation. CONCLUSION: This result suggested that a culture method with shaking can be applied to commercially available established cell lines to generate artificial islets, which might be used for a bioartificial pancreas | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 4225~4227 | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Culture Media | - |
dc.subject.MESH | Insulin/secretion* | - |
dc.subject.MESH | Microspheres* | - |
dc.title | Manufacturing of insulin-secreting spheroids with the RIN-5F cell line using a shaking culture method | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | D.J. Joo | - |
dc.contributor.googleauthor | J.Y. Kim | - |
dc.contributor.googleauthor | J.I. Lee | - |
dc.contributor.googleauthor | J.H. Jeong | - |
dc.contributor.googleauthor | Y. Cho | - |
dc.contributor.googleauthor | M.K. Ju | - |
dc.contributor.googleauthor | K.H. Huh | - |
dc.contributor.googleauthor | M.S. Kim | - |
dc.contributor.googleauthor | Y.S. Kim | - |
dc.identifier.doi | 10.1016/j.transproceed.2010.09.090 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00424 | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A00956 | - |
dc.contributor.localId | A03749 | - |
dc.contributor.localId | A03872 | - |
dc.contributor.localId | A03948 | - |
dc.contributor.localId | A03949 | - |
dc.contributor.localId | A04344 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 21168670 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0041134510015101 | - |
dc.contributor.alternativeName | Kim, Myoung Soo | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Kim, Joon Ye | - |
dc.contributor.alternativeName | Jeong, Jin Ho | - |
dc.contributor.alternativeName | Cho, Yu Ri | - |
dc.contributor.alternativeName | Joo, Dong Jin | - |
dc.contributor.alternativeName | Joo, Man Ki | - |
dc.contributor.alternativeName | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Soo | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Kim, Joon Ye | - |
dc.contributor.affiliatedAuthor | Jeong, Jin Ho | - |
dc.contributor.affiliatedAuthor | Cho, Yu Ri | - |
dc.contributor.affiliatedAuthor | Joo, Dong Jin | - |
dc.contributor.affiliatedAuthor | Joo, Man Ki | - |
dc.contributor.affiliatedAuthor | Huh, Kyu Ha | - |
dc.citation.volume | 42 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 4225 | - |
dc.citation.endPage | 4227 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.42(10) : 4225-4227, 2010 | - |
dc.identifier.rimsid | 49835 | - |
dc.type.rims | ART | - |
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