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PCR-linked reverse DNA hybridization using oligonucleotide-specific probes of rpoB for identification of Mycobacterium avium and Mycobacterium intracellulare.

Authors
 Sun-Joon Bai  ;  Jin-Seong Eum  ;  Young-Doo Park  ;  Sang-Ho Chung  ;  Yoon-Hoh Kook  ;  Seong-Karp Hong 
Citation
 JOURNAL OF MICROBIOLOGICAL METHODS, Vol.83(3) : 291-295, 2010 
Journal Title
 JOURNAL OF MICROBIOLOGICAL METHODS 
ISSN
 0167-7012 
Issue Date
2010
MeSH
Bacterial Proteins/genetics* ; Bacterial Typing Techniques/methods* ; DNA, Bacterial/genetics ; DNA-Directed RNA Polymerases/genetics* ; Humans ; Molecular Sequence Data ; Mycobacterium Infections/microbiology ; Mycobacterium avium/classification ; Mycobacterium avium/genetics ; Mycobacterium avium/isolation & purification* ; Mycobacterium avium Complex/classification ; Mycobacterium avium Complex/genetics ; Mycobacterium avium Complex/isolation & purification* ; Nucleic Acid Hybridization/methods* ; Oligonucleotide Probes/genetics ; Polymerase Chain Reaction/methods* ; Species Specificity
Keywords
Mycobacterium avium ; Mycobacterium intracellulare ; rpoB ; PCR-linked reverse DNA hybridization
Abstract
A PCR-linked reverse DNA hybridization method using two different specific rpoB DNA probes (Avp and Intp) of Mycobacterium avium and Mycobacterium intracellulare, respectively, were evaluated for the differentiation and identification of M. avium and M. intracellulare culture isolates. Among the 504 culture isolates tested by this method, 48 strains showed positive results for M. avium and 60 strains showed positive results for M. intracellulare. The other 396 culture isolates showed negative results for both M. avium and M. intracellulare. These results were consistent with those obtained from partial rpoB (306 bp) sequence analysis and biochemical tests. The negative strains obtained by this DNA hybridization method were identified as M. tuberculosis (366 strains), M. peregrinum (11 strains), M. abscessus (9 strains), M. fortuitum (8 strains), and M. flavescens (2 strains) by rpoB DNA sequence analysis. Due to the high sensitive and specific result obtained by this assay, we suggest that this PCR-linked reverse DNA hybridization method using two different specific rpoB DNA probes of M. avium and M. intracellulare would be used for the rapid and precise method for differentiation and identification of M. avium and M. intracellulare
Full Text
http://www.sciencedirect.com/science/article/pii/S0167701210003258
DOI
10.1016/j.mimet.2010.09.021
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Bai, Sun Joon(배선준) ORCID logo https://orcid.org/0000-0001-5027-3232
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102401
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