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Detection of Mycobacterium leprae by Real-time PCR Targeting Mycobacterium leprae-Specific Repetitive Element Sequence
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2015-04-23T17:23:00Z | - |
dc.date.available | 2015-04-23T17:23:00Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1226-0487 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102356 | - |
dc.description.abstract | Mycobacterium leprae detection is difficult even with molecular biological techniques due to the low sensitivity of current methodologies. In this report, real-time PCR targeting the M. leprae-specific repetitive element (RLEP) sequence was developed as a new diagnostic tool and evaluated using clinical specimens. For this, M. leprae DNAs were extracted from skin biopsy specimens from 80 patients and analyzed by real-time PCR using TaqMan probe. Then, the detection efficiency of the real-time PCR was compared with that of standard PCR. In brief, the rate of positive detection by the standard PCR and real-time PCR was 32.50% and 66.25%, respectively. The results seemed to clearly show that the TaqMan real-time PCR developed in this study may be a useful tool for sensitive detection of M. leprae from clinical specimens. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 127~131 | - |
dc.relation.isPartOf | Journal of Experimental & Biomedcal Science (대한의생명과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Detection of Mycobacterium leprae by Real-time PCR Targeting Mycobacterium leprae-Specific Repetitive Element Sequence | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology & Immunology (미생물학) | - |
dc.contributor.googleauthor | Hyunwoo Jin | - |
dc.contributor.googleauthor | Hye-Young Wang | - |
dc.contributor.googleauthor | Jong-Pill Kim | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.contributor.googleauthor | Hyeyoung Lee | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01408 | - |
dc.subject.keyword | Mycobacterium leprae | - |
dc.subject.keyword | Real-time PCR | - |
dc.subject.keyword | Repetitive element sequence (RLEP) | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.citation.volume | 16 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 127 | - |
dc.citation.endPage | 131 | - |
dc.identifier.bibliographicCitation | Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.16(2) : 127-131, 2010 | - |
dc.identifier.rimsid | 40881 | - |
dc.type.rims | ART | - |
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