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Pulsed electromagnetic field stimulates cellular proliferation in human intervertebral disc cells.

DC Field Value Language
dc.contributor.author김보람-
dc.contributor.author김향-
dc.contributor.author김호중-
dc.contributor.author문성환-
dc.contributor.author문은수-
dc.contributor.author박진오-
dc.contributor.author이환모-
dc.contributor.author권언혜-
dc.date.accessioned2015-04-23T17:19:33Z-
dc.date.available2015-04-23T17:19:33Z-
dc.date.issued2010-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/102248-
dc.description.abstractPURPOSE: The purpose of this study is to investigate the mechanism of cellular proliferation of electromagnetic field (EMF) on human intervertebral disc (IVD) cells. MATERIALS AND METHODS: Human IVD cells were cultured three-dimensionally in alginate beads. EMF was exposed to IVD cells with 650 Ω, 1.8 millitesla magnetic flux density, 60 Hz sinusoidal wave. Cultures were divided into a control and EMF group. Cytotoxicity, DNA synthesis and proteoglycan synthesis were measured by MTT assay, [(3)H]-thymidine, and [(35)S]-sulfate incorporation. To detect phenotypical expression, reverse transcription-polymerase chain reactions (RT-PCR) were performed for aggrecan, collagen type I, and type II mRNA expression. To assess action mechanism of EMF, IVD cells were exposed to EMF with N(G)-Monomethyl-L-arginine (NMMA) and acetylsalicylic acid (ASA). RESULTS: There was no cytotoxicity in IVD cells with the EMF group in MTT assay. Cellular proliferation was observed in the EMF group (p < 0.05). There was no difference in newly synthesized proteoglycan normalized by DNA synthesis between the EMF group and the control. Cultures with EMF showed no significant change in the expression of aggrecan, type I, and type II collagen mRNA compared to the control group. Cultures with NMMA (blocker of nitric oxide) or ASA (blocker of prostaglandin E2) exposed to EMF demonstrated decreased DNA synthesis compared to control cultures without NMMA or ASA (p < 0.05). CONCLUSION: EMF stimulated DNA synthesis in human IVD cells while no significant effect on proteoglycan synthesis and chondrogenic phenotype expressions. DNA synthesis was partially mediated by nitric oxide and prostaglandin E2. EMF can be utilized to stimulate proliferation of IVD cells, which may provide efficient cell amplification in cell therapy to degenerative disc disease-
dc.description.statementOfResponsibilityopen-
dc.format.extent954~959-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAspirin/pharmacology-
dc.subject.MESHCell Proliferation/radiation effects*-
dc.subject.MESHCollagen/metabolism-
dc.subject.MESHDinoprostone/metabolism-
dc.subject.MESHElectromagnetic Fields*-
dc.subject.MESHEnzyme Inhibitors/pharmacology-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHIntervertebral Disc/pathology*-
dc.subject.MESHIntervertebral Disc/radiation effects-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHNitric Oxide/metabolism-
dc.subject.MESHTetrazolium Salts/pharmacology-
dc.subject.MESHThiazoles/pharmacology-
dc.subject.MESHomega-N-Methylarginine/pharmacology-
dc.titlePulsed electromagnetic field stimulates cellular proliferation in human intervertebral disc cells.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentMedical Research Center (임상의학연구센터)-
dc.contributor.googleauthorHwan-Mo Lee-
dc.contributor.googleauthorUn-Hye Kwon-
dc.contributor.googleauthorHyang Kim-
dc.contributor.googleauthorHo-Joong Kim-
dc.contributor.googleauthorBoram Kim-
dc.contributor.googleauthorJin-Oh Park-
dc.contributor.googleauthorEun-Soo Moon-
dc.contributor.googleauthorSeong-Hwan Moon-
dc.identifier.doi10.3349/ymj.2010.51.6.954-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01186-
dc.contributor.localIdA00509-
dc.contributor.localIdA01106-
dc.contributor.localIdA01365-
dc.contributor.localIdA01372-
dc.contributor.localIdA01703-
dc.contributor.localIdA03333-
dc.contributor.localIdA00229-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid20879066-
dc.subject.keywordElectromagnetic fields-
dc.subject.keywordintervertebral disc-
dc.subject.keywordnitric oxide-
dc.subject.keywordprostaglandin E2-
dc.contributor.alternativeNameKim, Bo Ram-
dc.contributor.alternativeNameKim, Hyang-
dc.contributor.alternativeNameKim, Ho Joong-
dc.contributor.alternativeNameMoon, Seong Hwan-
dc.contributor.alternativeNameMoon, Eun Su-
dc.contributor.alternativeNamePark, Jin Oh-
dc.contributor.alternativeNameLee, Hwan Mo-
dc.contributor.alternativeNameKwon, Un Hye-
dc.contributor.affiliatedAuthorKim, Ho Joong-
dc.contributor.affiliatedAuthorKim, Bo Ram-
dc.contributor.affiliatedAuthorKim, Hyang-
dc.contributor.affiliatedAuthorMoon, Seong Hwan-
dc.contributor.affiliatedAuthorMoon, Eun Su-
dc.contributor.affiliatedAuthorPark, Jin Oh-
dc.contributor.affiliatedAuthorLee, Hwan Mo-
dc.contributor.affiliatedAuthorKwon, Un Hye-
dc.citation.volume51-
dc.citation.number6-
dc.citation.startPage954-
dc.citation.endPage959-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.51(6) : 954-959, 2010-
dc.identifier.rimsid51162-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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