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Stimulation of epidermal calcium gradient loss increases the expression of hyaluronan and CD44 in mouse skin

Authors
 S.-E. Lee  ;  J.-E. Jun  ;  E.-H. Choi  ;  S.-K. Ahn  ;  S.-H. Lee 
Citation
 CLINICAL AND EXPERIMENTAL DERMATOLOGY, Vol.35(6) : 650-657, 2010 
Journal Title
 CLINICAL AND EXPERIMENTAL DERMATOLOGY 
ISSN
 0307-6938 
Issue Date
2010
MeSH
Animals ; Calcium/metabolism* ; Epidermis/metabolism* ; Female ; Hyaluronan Receptors/metabolism* ; Hyaluronic Acid/metabolism* ; Interleukin-1/metabolism ; Mice ; Mice, Hairless ; Tumor Necrosis Factor-alpha/metabolism
Abstract
BACKGROUND: Hyaluronan (HA), a major extracellular matrix component in epidermis, has been found to accumulate in the epidermis after disruption of the epidermal barrier; however, the precise mechanisms underlying this process are not yet clear. Alterations in the epidermal calcium gradient are an important signal for permeability-barrier homeostasis. Thus, we hypothesized that epidermal calcium-ions might regulate HA expression. AIM: To investigate whether changes in the epidermal calcium gradient and subsequent induction of cytokines regulate HA, HA synthase (HAS) and HA receptor (CD44) expression in mouse epidermis, and to clarify the mechanisms of HA induction. METHODS: Sonophoresis of 1.5 mmol/L Ca(2+)-containing gel or Ca(2+)-free gel was performed to manipulate the epidermal Ca(2+) content without disrupting the permeability barrier. We also manipulated the Ca(2+) gradient by tape-stripping with or without 2 h immersion in 1.2 mmol/L Ca(2+)-containing solutions. Next we inhibited cytokine activity using tumour necrosis factor (TNF)-alpha or interleukin (IL)-1 inhibitors before sonophoresis. Six hours after each treatment, the expression of HA, HAS and CD44 were analysed using reverse transcription PCR and immunohistochemical stains. RESULTS: Sonophoresis of Ca(2+)-free gel significantly increased HA, HAS3 and CD44 expression in epidermis and in tape-stripped skin. However, the inhibition of Ca(2+) decrease in the upper epidermis by sonophoresis of Ca(2+)-containing gel or immersion of barrier-disrupted skin into a Ca(2+)-containing solution attenuated these inductions. Specific inhibitors of TNF-alpha and IL-1 specific inhibitors also abolished the sonophoresis-induced expression of HA, HAS3 and CD44. CONCLUSIONS: These results suggest that modulations in epidermal calcium regulate HA and CD44 expression directly or via induction of cytokines.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2230.2009.03699.x/abstract
DOI
10.1111/j.1365-2230.2009.03699.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sang Eun(이상은) ORCID logo https://orcid.org/0000-0003-4720-9955
Lee, Seung Hun(이승헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102207
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