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Cited 14 times in

The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression

DC FieldValueLanguage
dc.contributor.author박수진-
dc.contributor.author육종인-
dc.contributor.author이유미-
dc.contributor.author임승길-
dc.contributor.author최한석-
dc.contributor.author가디죠게스-
dc.date.accessioned2015-04-23T17:13:14Z-
dc.date.available2015-04-23T17:13:14Z-
dc.date.issued2010-
dc.identifier.issn8756-3282-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/102050-
dc.description.abstractOsteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1498~1507-
dc.relation.isPartOfBONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Differentiation/genetics-
dc.subject.MESHCell Differentiation/physiology*-
dc.subject.MESHCell Line-
dc.subject.MESHCell Proliferation-
dc.subject.MESHChromatin Immunoprecipitation-
dc.subject.MESHCore Binding Factor Alpha 1 Subunit/genetics-
dc.subject.MESHCore Binding Factor Alpha 1 Subunit/metabolism*-
dc.subject.MESHElectrophoretic Mobility Shift Assay-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHMice-
dc.subject.MESHMutagenesis, Site-Directed-
dc.subject.MESHOsteoblasts/cytology*-
dc.subject.MESHOsteoblasts/metabolism*-
dc.subject.MESHRNA Interference-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSnail Family Transcription Factors-
dc.subject.MESHTranscription Factors/genetics-
dc.subject.MESHTranscription Factors/metabolism*-
dc.titleThe transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSu Jin Park-
dc.contributor.googleauthorSeung-Hyun Jung-
dc.contributor.googleauthorGadi Jogeswar-
dc.contributor.googleauthorHyun-Mo Ryoo-
dc.contributor.googleauthorJong In Yook-
dc.contributor.googleauthorHan Seok Choi-
dc.contributor.googleauthorYumie Rhee-
dc.contributor.googleauthorCheol-Hee Kim-
dc.contributor.googleauthorSung-Kil Lim-
dc.identifier.doi10.1016/j.bone.2010.02.027-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02536-
dc.contributor.localIdA03012-
dc.contributor.localIdA03375-
dc.contributor.localIdA04206-
dc.contributor.localIdA00001-
dc.contributor.localIdA01540-
dc.relation.journalcodeJ00381-
dc.identifier.eissn1873-2763-
dc.identifier.pmid20215006-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S8756328210004874-
dc.subject.keywordSnail-
dc.subject.keywordRunx2-
dc.subject.keywordE-box-
dc.subject.keywordOsteogenic differentiation-
dc.contributor.alternativeNamePark, Su Jin-
dc.contributor.alternativeNameYook, Jong In-
dc.contributor.alternativeNameRhee, Yumie-
dc.contributor.alternativeNameLim, Sung Kil-
dc.contributor.alternativeNameChoi, Han Seok-
dc.contributor.alternativeNameGadi, Jogeswar-
dc.contributor.affiliatedAuthorYook, Jong In-
dc.contributor.affiliatedAuthorRhee, Yumie-
dc.contributor.affiliatedAuthorLim, Sung Kil-
dc.contributor.affiliatedAuthorChoi, Han Seok-
dc.contributor.affiliatedAuthorGadi, Jogeswar-
dc.contributor.affiliatedAuthorPark, Su Jin-
dc.citation.volume46-
dc.citation.number6-
dc.citation.startPage1498-
dc.citation.endPage1507-
dc.identifier.bibliographicCitationBONE, Vol.46(6) : 1498-1507, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Endocrinology (내분비연구소) > 1. Journal Papers

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