Cited 8 times in
Illumina-microarray analysis of mycophenolic acid-induced cell death in an insulin-producing cell line and primary rat islet cells: new insights into apoptotic pathways involved
DC Field | Value | Language |
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dc.contributor.author | 김유선 | - |
dc.contributor.author | 박윤종 | - |
dc.contributor.author | 조유리 | - |
dc.contributor.author | 주동진 | - |
dc.contributor.author | 주만기 | - |
dc.date.accessioned | 2015-04-23T17:10:56Z | - |
dc.date.available | 2015-04-23T17:10:56Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101977 | - |
dc.description.abstract | Mycophenolic acid (MPA), widely used to prevent organ transplant rejection, may induce toxicity and impair function in beta-cells. Mechanisms of MPA-induced cell death have not been fully explored. In this study, we examined gene expression patterns in INS-1E cells and isolated primary rat islets following MPA treatment using the Illumina-cDNA microarray. The MPA treatment decreases RhoGDI-alpha gene expression, which points to apoptosis by JNK activation through a MAPKs-dependent pathway. A strong association between RhoGDI-alpha and Rac1 activation during MPA-induced apoptosis is also consistent with apoptosis through JNK. Suppression of RhoGDI-alpha using siRNA and gene over-expression both affected the cell death rate, consistent with Rac1 activation and downstream activation of MAPKs signaling. We confirmed that Rac1 protein mediates the interaction between RhoGDI-alpha and JNK signaling. We conclude that MPA-induced cell death in primary beta-cells and an insulin-secreting cell line proceeds through RhoGDI-alpha down-regulation linked to Rac1 activation, with subsequent activation of JNK. The RhoGDI-alpha/Rac1/JNK pathway may present a key to intervention in MPA-induced islet apoptosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1773~1782 | - |
dc.relation.isPartOf | CELLULAR SIGNALLING | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Guanine Nucleotide Dissociation Inhibitors/genetics | - |
dc.subject.MESH | Guanine Nucleotide Dissociation Inhibitors/metabolism | - |
dc.subject.MESH | Insulin/metabolism | - |
dc.subject.MESH | Insulin-Secreting Cells/drug effects | - |
dc.subject.MESH | Insulin-Secreting Cells/metabolism* | - |
dc.subject.MESH | Islets of Langerhans/drug effects | - |
dc.subject.MESH | Islets of Langerhans/metabolism* | - |
dc.subject.MESH | JNK Mitogen-Activated Protein Kinases/metabolism | - |
dc.subject.MESH | MAP Kinase Signaling System | - |
dc.subject.MESH | Mycophenolic Acid/toxicity* | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | rac1 GTP-Binding Protein/metabolism | - |
dc.subject.MESH | rho-Specific Guanine Nucleotide Dissociation Inhibitors | - |
dc.title | Illumina-microarray analysis of mycophenolic acid-induced cell death in an insulin-producing cell line and primary rat islet cells: new insights into apoptotic pathways involved | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Yun-Jong Park | - |
dc.contributor.googleauthor | Hyung Joon Ahn | - |
dc.contributor.googleauthor | Yu Seun Kim | - |
dc.contributor.googleauthor | Yuri Cho | - |
dc.contributor.googleauthor | Dong Jin Joo | - |
dc.contributor.googleauthor | Man Ki Ju | - |
dc.identifier.doi | 10.1016/j.cellsig.2010.07.005 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A01603 | - |
dc.contributor.localId | A03872 | - |
dc.contributor.localId | A03948 | - |
dc.contributor.localId | A03949 | - |
dc.relation.journalcode | J00502 | - |
dc.identifier.eissn | 1873-3913 | - |
dc.identifier.pmid | 20637860 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0898656810001981 | - |
dc.subject.keyword | Mycophenolic acid | - |
dc.subject.keyword | INS-1E | - |
dc.subject.keyword | Islet cells | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | RhoGDI-α | - |
dc.subject.keyword | Rac1 | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Park, Yun Jong | - |
dc.contributor.alternativeName | Cho, Yu Ri | - |
dc.contributor.alternativeName | Joo, Dong Jin | - |
dc.contributor.alternativeName | Joo, Man Ki | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Park, Yun Jong | - |
dc.contributor.affiliatedAuthor | Cho, Yu Ri | - |
dc.contributor.affiliatedAuthor | Joo, Dong Jin | - |
dc.contributor.affiliatedAuthor | Joo, Man Ki | - |
dc.citation.volume | 22 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1773 | - |
dc.citation.endPage | 1782 | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, Vol.22(11) : 1773-1782, 2010 | - |
dc.identifier.rimsid | 50987 | - |
dc.type.rims | ART | - |
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