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Dietary polyphenols affect MUC5AC expression and ciliary movement in respiratory cells and nasal mucosa

Authors
 Chang, Jung Hyun  ;  Song, Kee Jae  ;  Kim, Hyun-Jun  ;  Kim, Jeong Hong  ;  Kim, Na Hyun  ;  Kim, Kyung-Su 
Citation
 AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, Vol.24(2) : 59-62, 2010 
Journal Title
AMERICAN JOURNAL OF RHINOLOGY & ALLERGY
ISSN
 1945-8924 
Issue Date
2010
MeSH
Catechin/administration & dosage ; Catechin/analogs & derivatives ; Catechols/administration & dosage ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Curcumin/administration & dosage ; Diet ; Down-Regulation ; Fatty Alcohols/administration & dosage ; Humans ; Interleukin-1beta/immunology ; Interleukin-1beta/metabolism ; Mucin 5AC/genetics ; Mucin 5AC/metabolism* ; Mucociliary Clearance/drug effects* ; Nasal Mucosa/metabolism ; Nasal Mucosa/pathology ; Quercetin/administration & dosage ; Quercetin/analogs & derivatives ; Respiratory Mucosa/drug effects* ; Respiratory Mucosa/immunology ; Respiratory Mucosa/metabolism ; Respiratory Mucosa/pathology
Keywords
Anti-hypersecretion ; ciliary beat frequency ; human nasal mucosa ; MUC5AC ; mucin ; polyphenol ; respiratory cells
Abstract
BACKGROUND: Dietary polyphenols have been widely consumed in food, and their anti-inflammatory and anticancer activities have been recently studied. Although the effects of dietary polyphenols on mucin hypersecretion have been studied to some extent, the effects of mucociliary movement have not been elucidated thus far. Therefore, we investigated whether dietary polyphenols inhibit MUC5AC gene expression in NCI-H292 cells, and, if so, whether they would have an effect on ciliary beat frequency (CBF) of human nasal mucosa.

METHODS: NCI-H292 cells were pretreated with four different dietary polyphenols ([6]-gingerol, epigallocatechin gallate (EGCG), curcumin, and quercetin) and were treated with IL-1beta (10 ng/mL). Proliferation of NCI-H292 cells was analyzed. The mRNA expression of MUC5AC was determined by real-time polymerase chain reaction. CBF of normal nasal mucosa, which was obtained from the ethmoid sinus and treated with the polyphenols, was assessed via inverted microscope and computerized program.

RESULTS: Minimal inhibitory concentration (MIC) of MUC5AC expression of each polyphenol was found as follows: [6]-gingerol, 1 microM; EGCG, 20 microM; quercetin, 40 microM; and curcumin, 10 microM. No polyphenol influenced cell proliferation at this MIC. CBF was not affected by [6]-gingerol, quercetin, or EGCG, but ciliary movement decreased in curcumin.

CONCLUSION: [6]-Gingerol, quercetin, and EGCG may be considered as antihypersecretory agents because they effectively inhibit mucus secretion of respiratory epithelial cells while maintaining normal nasal ciliary movement.
Full Text
http://www.ingentaconnect.com/content/ocean/ajra/2010/00000024/00000002/art00001
DOI
10.2500/ajra.2010.24.3447
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101824
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