Cited 6 times in
Decisive role of apurinic/apyrimidinic endonuclease/Ref-1 in initiation of cell death
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김경환 | - |
dc.contributor.author | 김현우 | - |
dc.contributor.author | 김현정 | - |
dc.contributor.author | 박수철 | - |
dc.contributor.author | 조경주 | - |
dc.date.accessioned | 2015-04-23T17:05:44Z | - |
dc.date.available | 2015-04-23T17:05:44Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1044-7431 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101811 | - |
dc.description.abstract | The apurinic/apyrimidinic endonuclease/redox effector factor-1 (APE/Ref-1) is involved in the base excision repair of apurinic/apyrimidinic sites induced by oxidative DNA damage. APE/Ref-1 was decreased by kainic acid (KA) injury in a time-dependent manner at the level of proteins, not transcripts. We investigated whether alteration of APE/Ref-1 amounts would influence hippocampal cell fate, survival or death, after KA injury. Overexpression of APE/Ref-1 using adenovirus and restoration of APE small peptides significantly reduced KA-induced hippocampal cell death. Both silencing of APE/Ref-1 by siRNA and inhibition of endonuclease by an antibody significantly increased caspase-3 activity and apoptotic cell death triggered from the early time after exposure to KA. These findings suggest that cell death is initiated by reducing APE/Ref-1 protein and inhibiting its repair function in spite of enough protein amounts. In conclusion, APE/Ref-1 may be a regulator of cell death initiation, and APE small peptides could provide molecular mechanism-based therapies for neuroprotection in progressive excitotoxic neuronal damage. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 267~276 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR NEUROSCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/genetics | - |
dc.subject.MESH | Adenoviridae/metabolism | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | CellDeath/physiology* | - |
dc.subject.MESH | DNA Damage | - |
dc.subject.MESH | DNA Repair | - |
dc.subject.MESH | DNA-(ApurinicorApyrimidinicSite) Lyase/genetics | - |
dc.subject.MESH | DNA-(ApurinicorApyrimidinicSite) Lyase/metabolism* | - |
dc.subject.MESH | Hippocampus/cytology | - |
dc.subject.MESH | Hippocampus/pathology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kainic Acid/pharmacology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neurons/drug effects | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Neurons/pathology | - |
dc.subject.MESH | Peptides/genetics | - |
dc.subject.MESH | Peptides/metabolism | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.title | Decisive role of apurinic/apyrimidinic endonuclease/Ref-1 in initiation of cell death | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Kyoung Joo Cho | - |
dc.contributor.googleauthor | Hyun Jeong Kim | - |
dc.contributor.googleauthor | Soo Chul Park | - |
dc.contributor.googleauthor | Hyun Woo Kim | - |
dc.contributor.googleauthor | Gyung Whan Kim | - |
dc.identifier.doi | 10.1016/j.mcn.2010.07.001 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00310 | - |
dc.contributor.localId | A01543 | - |
dc.contributor.localId | A03804 | - |
dc.contributor.localId | A01130 | - |
dc.contributor.localId | A01125 | - |
dc.relation.journalcode | J02245 | - |
dc.identifier.eissn | 1095-9327 | - |
dc.identifier.pmid | 20637286 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S104474311000151X | - |
dc.subject.keyword | APE/Ref-1 | - |
dc.subject.keyword | Adenovirus | - |
dc.subject.keyword | siRNA | - |
dc.subject.keyword | APE small peptide | - |
dc.subject.keyword | TAT-mediated transduction | - |
dc.subject.keyword | Neutral antibody | - |
dc.contributor.alternativeName | Kim, Gyung Whan | - |
dc.contributor.alternativeName | Kim, Hyun Woo | - |
dc.contributor.alternativeName | Kim, Hyun Jeong | - |
dc.contributor.alternativeName | Park, Soo Chul | - |
dc.contributor.alternativeName | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Gyung Whan | - |
dc.contributor.affiliatedAuthor | Park, Soo Chul | - |
dc.contributor.affiliatedAuthor | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Jeong | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Woo | - |
dc.citation.volume | 45 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 267 | - |
dc.citation.endPage | 276 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR NEUROSCIENCE, Vol.45(3) : 267-276, 2010 | - |
dc.identifier.rimsid | 54622 | - |
dc.type.rims | ART | - |
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