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16S rRNA 및 Internal Transcribed Spacer 염기서열 분석법을 이용한 세균 및 진균 동정

Other Titles
 Identification of Bacterial and Fungal Isolates by Sequence Analysis of 16S rRNA and Internal Transcribed Spacer 
Authors
 Younhee Park  ;  Hee Bong Shin  ;  Chang Ki Kim  ;  Kyoung Ho Roh  ;  Jong Hwa Yum  ;  Dongeun Yong  ;  Seok Hoon Jeong  ;  Kyungwon Lee 
Citation
 Korean Journal of Clinical Microbiology (대한임상미생물학회지), Vol.13(1) : 34-39, 2010 
Journal Title
 Korean Journal of Clinical Microbiology (대한임상미생물학회지) 
ISSN
 1229-0025 
Issue Date
2010
Keywords
16S rRNA ; Internal transcribed spacer ; Nucleotide sequence ; Bacterial identification
Abstract
BACKGROUND: Accurate and rapid identification of pathogens is one of the most important tasks of the clinical microbiology laboratory, and, in cases of rare pathogens, the identification is difficult and time-consuming upon the use of conventional methods alone. Herein, we will report our molecular work involving the identification of bacteria and fungi. METHODS: Sixty bacterial isolates had been collected from November 2004 to May 2007, and 15 fungal isolates had been collected from September 2005 to May 2007. Species identifications were performed using sequence analyses of the 16S rRNA region of bacteria and the internal transcribed spacer (ITS) region of fungi. The data were compared with those of GenBank (http://www.ncbi.nlm.nih.gov/) or EMBL (http://www.ebi.ac.uk/embl/). RESULTS: Sixty bacterial isolates included: 23 isolates with genus information (group 1), 17 isolates (group 2) that were too fastidious for genus or species identification, 16 isolates (group 3) with results from identification kits having low confidence, and 4 isolates (group 4) with odd antibiograms according to the species. In 58 of 60 isolates, identification of the genus or species could be obtained using molecular genetic methods. Thirty-eight isolates (63%) and 20 (33%) of 58 isolates could be identified at the species and genus levels, repectively. Among the total of 15 fungal isolates, 11 (73%) and 4 (27%) isolates were identified at the species and genus levels, respectively. CONCLUSION: 16S rRNA and ITS sequencing analyses are very useful for identifying the species or genus of a pathogenic microorganism in the clinical microbiology laboratory
Files in This Item:
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DOI
10.5145/KJCM.2010.13.1.34
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Ki(김창기)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101766
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