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Prevalence and resistance patterns of extended-spectrum and AmpC β-lactamase in Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Salmonella serovar Stanley in a Korean tertiary hospital

Authors
 SOON DEOK PARK  ;  YOUNG UH  ;  GYUSANG LEE  ;  KWANHUN LIM  ;  JONG BAE KIM  ;  SEOK HOON JEONG 
Citation
 APMIS, Vol.118(10) : 801-808, 2010 
Journal Title
 APMIS 
ISSN
 0903-4641 
Issue Date
2010
MeSH
Bacterial Proteins/biosynthesis ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism* ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Escherichia coli/drug effects ; Escherichia coli/enzymology* ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Gram-Negative Bacterial Infections/microbiology ; Humans ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/enzymology* ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/isolation & purification ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Prevalence ; Proteus mirabilis/drug effects ; Proteus mirabilis/enzymology* ; Proteus mirabilis/genetics ; Proteus mirabilis/isolation & purification ; Republic of Korea ; Salmonella/drug effects ; Salmonella/enzymology* ; Salmonella/genetics ; Salmonella/isolation & purification ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics ; beta-Lactamases/metabolism*
Keywords
Extended‐spectrum β‐lactamase ; AmpC β‐lactamase ; boronic acid ; polymerase chain reaction ; pulsed‐field gel electrophoresis
Abstract
A total of 100 clinical isolates of Escherichia coli (n = 35), Klebsiella pneumoniae (n = 63), Proteus mirabilis (n = 1), and Salmonella serovar Stanley (n = 1), showing resistance to cefoxitin, or returning positive in extended-spectrum β-lactamase (ESBL) by Clinical and Laboratory Standards Institute (CLSI) ESBL confirmatory method, were studied. The isolates were examined by the boronic acid (BA) disk test, polymerase chain reaction, and pulsed-field gel electrophoresis (PFGE) to investigate genetic similarities. The concurrence rates for ESBLs by the CLSI and the BA disk test were 97% for E. coli and 96.7% for K. pneumoniae. A total of 41 isolates showing cefoxitin resistance yielded all positive by the BA disk test. All the 33 K. pneumoniae isolates, which showed positive by the BA disk test, were carrying AmpC genes. The TEM and CTX-M types were predominant in E. coli and the SHV and the CIT and/or DHA types were predominant in K. pneumoniae. PFGE analysis showed almost 75% of genetic similarities among K. pneumoniae isolates producing ESBLs and/or AmpC β-lactamases (AmpCs) as each K. pneumoniae carried variable genes and showed variable antibiotic patterns. Clearly, the BA disk test was a useful method for the detection of ESBLs and AmpCs. In particular, cefoxitin resistance and BA-positive trait of K. pneumoniae do reflect the presence of AmpC genes in the organism.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0463.2010.02663.x/abstract
DOI
10.1111/j.1600-0463.2010.02663.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101726
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