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Bifidobacterium lactis inhibits NF-kappaB in intestinal epithelial cells and prevents acute colitis and colitis-associated colon cancer in mice

DC Field Value Language
dc.contributor.author이상길-
dc.contributor.author천재희-
dc.contributor.author김원호-
dc.contributor.author김태일-
dc.contributor.author박재준-
dc.contributor.author김승원-
dc.date.accessioned2015-04-23T16:56:49Z-
dc.date.available2015-04-23T16:56:49Z-
dc.date.issued2010-
dc.identifier.issn1078-0998-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101532-
dc.description.abstractBACKGROUND: The aim of this study was to investigate the antiinflammatory effects of Bifidobacterium lactis on intestinal epithelial cells (IECs) and on experimental acute murine colitis and its tumor prevention effects on colitis-associated cancer (CAC) in mice. METHODS: Human HT-29 cells were stimulated with IL-1beta, lipopolysaccharides, or tumor necrosis factor-alpha with and without B. lactis, and the effects of B. lactis on nuclear factor kappa B (NF-kappaB) signaling in IEC were examined. For in vivo study, dextran sulfate sodium (DSS)-treated mice were fed with and without B. lactis. Finally, we induced colonic tumors in mice by azoxymethane (AOM) and DSS and evaluated the effects of B. lactis on tumor growth. RESULTS: B. lactis significantly suppressed NF-kappaB activation, including NF-kappaB-binding activity and NF-kappaB-dependent reporter gene expression in a dose-dependent manner, and suppressed IkappaB-alpha degradation, which correlated with the downregulation of NF-kappaB-dependent gene products. Moreover, B. lactis suppressed the development of acute colitis in mice. Compared with the DSS group, the severity of DSS-induced colitis as assessed by disease activity index, colon length, and histological score was reduced in the B. lactis-treated group. In the CAC model, the mean number and size of tumors in the B. lactis-treated group were significantly lower than those in the AOM group. CONCLUSIONS: Our data demonstrate that B. lactis inhibits NF-kappaB and NF-kappaB-regulated genes in IEC and prevents acute colitis and CAC in mice. These results suggest that B. lactis could be a potential preventive agent for CAC as well as a therapeutic agent for inflammatory bowel disease.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfINFLAMMATORY BOWEL DISEASES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcute Disease-
dc.subject.MESHAnimals-
dc.subject.MESHAzoxymethane/toxicity-
dc.subject.MESHBifidobacteriales Infections/prevention & control-
dc.subject.MESHBifidobacterium/physiology*-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCarcinogens/toxicity-
dc.subject.MESHChronic Disease-
dc.subject.MESHColitis/chemically induced-
dc.subject.MESHColitis/complications-
dc.subject.MESHColitis/prevention & control*-
dc.subject.MESHColon/metabolism-
dc.subject.MESHColon/microbiology-
dc.subject.MESHColon/pathology-
dc.subject.MESHColonic Neoplasms/chemically induced-
dc.subject.MESHColonic Neoplasms/complications-
dc.subject.MESHColonic Neoplasms/prevention & control*-
dc.subject.MESHDextran Sulfate/toxicity-
dc.subject.MESHElectrophoretic Mobility Shift Assay-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHEpithelial Cells/metabolism-
dc.subject.MESHEpithelial Cells/microbiology*-
dc.subject.MESHEpithelial Cells/pathology-
dc.subject.MESHImmunoenzyme Techniques-
dc.subject.MESHInflammation/chemically induced-
dc.subject.MESHInflammation/complications-
dc.subject.MESHInflammation/prevention & control-
dc.subject.MESHIntestinal Mucosa/metabolism-
dc.subject.MESHIntestinal Mucosa/microbiology*-
dc.subject.MESHIntestinal Mucosa/pathology-
dc.subject.MESHLipopolysaccharides/pharmacology-
dc.subject.MESHLuciferases/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHNF-kappa B/antagonists & inhibitors*-
dc.subject.MESHNF-kappa B/metabolism-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.titleBifidobacterium lactis inhibits NF-kappaB in intestinal epithelial cells and prevents acute colitis and colitis-associated colon cancer in mice-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSeung Won Kim-
dc.contributor.googleauthorHee Man Kim-
dc.contributor.googleauthorKyoung Min Yang-
dc.contributor.googleauthorSun-Ah Kim-
dc.contributor.googleauthorSung-Kyu Kim-
dc.contributor.googleauthorMin Ji An-
dc.contributor.googleauthorJae Jun Park-
dc.contributor.googleauthorSang Kil Lee-
dc.contributor.googleauthorTae Il Kim-
dc.contributor.googleauthorWon Ho Kim-
dc.contributor.googleauthorJae Hee Cheon-
dc.identifier.doi10.1002/ibd.21262-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02812-
dc.contributor.localIdA00774-
dc.contributor.localIdA01079-
dc.contributor.localIdA01636-
dc.contributor.localIdA04030-
dc.relation.journalcodeJ01060-
dc.identifier.eissn1536-4844-
dc.identifier.pmid20310012-
dc.identifier.urlhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00054725-201009000-00012&LSLINK=80&D=ovft-
dc.contributor.alternativeNameLee, Sang Kil-
dc.contributor.alternativeNameCheon, Jae Hee-
dc.contributor.alternativeNameKim, Won Ho-
dc.contributor.alternativeNameKim, Tae Il-
dc.contributor.alternativeNamePark, Jae Jun-
dc.contributor.affiliatedAuthorLee, Sang Kil-
dc.contributor.affiliatedAuthorKim, Won Ho-
dc.contributor.affiliatedAuthorKim, Tae Il-
dc.contributor.affiliatedAuthorPark, Jae Jun-
dc.contributor.affiliatedAuthorCheon, Jae Hee-
dc.citation.volume16-
dc.citation.number9-
dc.citation.startPage1514-
dc.citation.endPage1525-
dc.identifier.bibliographicCitationINFLAMMATORY BOWEL DISEASES, Vol.16(9) : 1514-1525, 2010-
dc.identifier.rimsid47799-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers

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