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The Roles of Reactive Oxygen Species Produced by Contact Allergens and Irritants in Monocyte-derived Dendritic Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민걸 | - |
dc.contributor.author | 제정환 | - |
dc.date.accessioned | 2015-04-23T16:55:23Z | - |
dc.date.available | 2015-04-23T16:55:23Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1013-9087 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101485 | - |
dc.description.abstract | BACKGROUND: Although reactive oxygen species (ROS) have been produced in both mouse bone marrow-derived dendritic cells (DCs) and XS-106 DCs by contact sensitizers and irritants in previous studies, the generation of ROS in human monocyte-derived DCs (MoDCs) and their role in contact hypersensitivity (CHS) has yet to be elucidated. OBJECTIVE: The purpose of this study was to determine whether contact allergens and irritants induce ROS in MoDCs and, if so, to evaluate the role of contact allergen and irritant induced-ROS in MoDCs in CHS. METHODS: Production of ROS was measured by 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H(2)DCFDA) assay. Surface CD86 and HLA-DR molecules were detected by flow cytometry. Protein carbonylation was detected by Western blotting. RESULTS: ROS were produced by contact allergens such as dinitrochlorobenzene (DNCB) and thimerosal and the irritant benzalkonium chloride (BKC). DNCB-induced, but not BKC-induced, ROS increased surface CD86 and HLA-DR molecules on MoDCs and induced protein carbonylation. These changes were reduced in the presence of antioxidant N-acetyl cysteine. CONCLUSION: Our results suggest that DNCB-induced ROS may be different from those induced by irritant BKC. The DNCB-induced ROS may be associated with the CHS response, because they activate surface molecules on DCs that are important for generating immune reactions | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 269~278 | - |
dc.relation.isPartOf | ANNALS OF DERMATOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | The Roles of Reactive Oxygen Species Produced by Contact Allergens and Irritants in Monocyte-derived Dendritic Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | Dashlkhumbe Byamba | - |
dc.contributor.googleauthor | Tae Gyun Kim | - |
dc.contributor.googleauthor | Dong Hyun Kim | - |
dc.contributor.googleauthor | Jeong Hwan Je | - |
dc.contributor.googleauthor | Min-Geol Lee | - |
dc.identifier.doi | 10.5021/ad.2010.22.3.269 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02779 | - |
dc.contributor.localId | A03797 | - |
dc.relation.journalcode | J00158 | - |
dc.identifier.eissn | 2005-3894 | - |
dc.identifier.pmid | 20711262 | - |
dc.subject.keyword | Contact allergen | - |
dc.subject.keyword | Contact dermatitis | - |
dc.subject.keyword | Monocyte-derived dendritic cell | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.contributor.alternativeName | Lee, Min Geol | - |
dc.contributor.alternativeName | Je, Jeong Hwan | - |
dc.contributor.affiliatedAuthor | Lee, Min Geol | - |
dc.contributor.affiliatedAuthor | Je, Jeong Hwan | - |
dc.citation.volume | 22 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 269 | - |
dc.citation.endPage | 278 | - |
dc.identifier.bibliographicCitation | ANNALS OF DERMATOLOGY, Vol.22(3) : 269-278, 2010 | - |
dc.identifier.rimsid | 47762 | - |
dc.type.rims | ART | - |
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