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Changes in Allergenicity of Porcine Serum Albumin by Gamma Irradiation

Authors
 Koth-Bong-Woo-Ri Kim  ;  So-Young Lee  ;  Eu-Jin Song  ;  Jin-Gyu Park  ;  Ju-Woon Lee  ;  Myung-Woo Byun  ;  Kyu-Earn Kim  ;  Dong-Hyun Ahn 
Citation
 Journal of Korean Society for Food Science of Animal Resources (한국축산식품학회지), Vol.30(3) : 397-402, 2010 
Journal Title
Journal of Korean Society for Food Science of Animal Resources(한국축산식품학회지)
ISSN
 1225-8563 
Issue Date
2010
Keywords
porcine serum albumin ; allergenicity ; gamma irradiation
Abstract
Pork is an excellent source of essential nutrients such as protein. However, pork can trigger hypersensitivity and serum albumin of pork is known as major allergen. In this study, to evaluate the effect of gamma irradiation on the allergenicity of porcine serum albumin (PSA), PSA solution was irradiated at 3, 5, 7, 10, 15, and 20 kGy. The changes in the ability of PSA to bind IgG and patient's serum caused by gamma irradiation were observed by ci-ELISA and immunoblotting. SDS-PAGE was used for measuring the conformational change of gamma-irradiated PSA. The ability of 3-kGy-irradiated PSA to bind p-IgG and patient's serum was decreased to 30% and 15%, respectively. The binding ability showed no significant differences among all irradiated samples. SDS-PAGE showed that the irradiated PSA bands were degraded and aggregated. Immunoblotting of irradiated PSA revealed that IgG and patient's serum were rarely recognized at 3 kGy. Therefore, gamma irradiation could be applied to less-allergenic pork products
Files in This Item:
T201002121.pdf Download
DOI
10.5851/kosfa.2010.30.3.397
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101403
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