Cited 45 times in
Tumor suppression by apoptotic and anti-angiogenic effects of mortalin-targeting adeno-oncolytic virus
DC Field | Value | Language |
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dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2015-04-23T16:52:09Z | - |
dc.date.available | 2015-04-23T16:52:09Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1099-498X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101381 | - |
dc.description.abstract | BACKGROUND: Adeno-oncolytic (Adon) viruses offer an effective cancer therapeutic tool with several advantages, including wide host cell permeability, high transduction efficiency, safety, tumor selectivity, non-invasiveness, high genetic modifiability and high level of expression of the integrated transgenes. Armed Adon viruses in which the therapeutic efficacy of virus is enhanced by their coupling with cytotoxic, anti-angiogenic or anti-vascular gene products have gained importance because they engage additional mechanisms for tumor cell killing. In the present study, we selected mortalin, a stress chaperone that is tightly involved in human carcinogenesis, constructed a mortalin-targeting Adon (mot-Adon) virus and examined its therapeutic potential both in vitro and in vivo. METHODS: Mortalin-targeting plasmid and viral vectors that harbored mortalin-specific small interfering RNA sequences were constructed. The therapeutic value of these vectors was investigated in vitro and in vivo by cell culture and nude mice tumor models. RESULTS: We demonstrate that the mot-Adon virus has selective cytotoxicity for human cancer cells in vitro. Retrovirus-mediated overexpression of mortalin protected the cells against mot-Adon virus, confirming that mortalin silencing was the real cause of cancer cell death. Although mortalin overexpression enhanced malignant properties of cancer cells in breast xenograft models, mot-Adon virus elicited an enhanced anti-tumor effect. Immunohistochemical examination of the tumors showed that the mot-Adon virus caused enhanced apoptosis (mediated by reactivation of p53) and suppression of microvessel formation. CONCLUSIONS: Mortalin is up-regulated in a large variety of tumors and hence mot-Adon virus is proposed as a candidate cancer therapeutic agent | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 586~595 | - |
dc.relation.isPartOf | JOURNAL OF GENE MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/metabolism | - |
dc.subject.MESH | Angiogenesis Inhibitors/metabolism* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cytoprotection | - |
dc.subject.MESH | HSP70 Heat-Shock Proteins/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neoplasms/blood supply* | - |
dc.subject.MESH | Neoplasms/pathology | - |
dc.subject.MESH | Neoplasms/therapy* | - |
dc.subject.MESH | Oncolytic Virotherapy/methods* | - |
dc.subject.MESH | Oncolytic Viruses/metabolism* | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Tumor Suppressor Protein p53/metabolism | - |
dc.subject.MESH | Xenograft Model Antitumor Assays | - |
dc.title | Tumor suppression by apoptotic and anti-angiogenic effects of mortalin-targeting adeno-oncolytic virus | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute for Cancer Research (암연구소) | - |
dc.contributor.googleauthor | Ji Young Yoo | - |
dc.contributor.googleauthor | Jihoon Ryu | - |
dc.contributor.googleauthor | Ran Gao | - |
dc.contributor.googleauthor | Tomoko Yaguchi | - |
dc.contributor.googleauthor | Sunil C. Kaul | - |
dc.contributor.googleauthor | Renu Wadhwa | - |
dc.contributor.googleauthor | Chae-Ok Yun | - |
dc.identifier.doi | 10.1002/jgm.1471 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02614 | - |
dc.relation.journalcode | J01419 | - |
dc.identifier.eissn | 1521-2254 | - |
dc.identifier.pmid | 20603860 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jgm.1471/abstract | - |
dc.subject.keyword | adeno-oncolytic viruses | - |
dc.subject.keyword | angiogenesis | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | cancer therapy | - |
dc.subject.keyword | mortalin | - |
dc.subject.keyword | siRNA | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.citation.volume | 12 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 586 | - |
dc.citation.endPage | 595 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENE MEDICINE, Vol.12(7) : 586-595, 2010 | - |
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