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Effects of an oral adsorbent on oxidative stress and fibronectin expression in experimental diabetic nephropathy

DC FieldValueLanguage
dc.contributor.author강신욱-
dc.contributor.author곽승재-
dc.contributor.author김도희-
dc.contributor.author김승혜-
dc.contributor.author남보영-
dc.contributor.author박정탁-
dc.contributor.author유태현-
dc.contributor.author이금희-
dc.contributor.author이순하-
dc.contributor.author장태익-
dc.contributor.author한대석-
dc.contributor.author한승혁-
dc.date.accessioned2015-04-23T16:51:35Z-
dc.date.available2015-04-23T16:51:35Z-
dc.date.issued2010-
dc.identifier.issn0931-0509-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101363-
dc.description.abstractBACKGROUND: Previous studies have demonstrated that AST-120 (Kremezin((R))), a well-known oral adsorbent, inhibits the progression of diabetic (DM) and non-DM chronic kidney disease along with a decrease in oxidative stress. This study was undertaken to investigate whether AST-120 could reduce oxidative stress and ameliorate the development of nephropathy in experimental DM rats with normal renal function. METHODS: Rats were injected with diluent (C, n = 16) or 65 mg/kg streptozotocin intraperitoneally (DM, n = 16), and eight rats from each group were treated with chow containing 5% AST-120. After 3 months, plasma advanced oxidation protein products (AOPP) and total malondialdehyde (MDA) levels, 24-h urinary albumin excretion, and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) excretion were determined by ELISA. Glomerular endothelial nitric oxide synthase (eNOS), subunits of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (gp91phox, p47phox and p22phox), and fibronectin (FN) mRNA and protein expressions were determined by real-time PCR and western blot, respectively. In addition, dichlorodihydrofluorescein diacetate (DCF-DA) staining was performed to detect glomerular reactive oxygen species (ROS) production. RESULTS: Compared to the C group, 24-h urinary albumin excretion was significantly higher in the DM group (P < 0.01), and AST-120 treatment significantly reduced albuminuria in DM rats (P < 0.05). Glomerular eNOS, gp91phox, p47phox and FN expression were significantly increased in DM rats compared to C rats, and these increases in DM glomeruli were significantly abrogated by AST-120 treatment (P < 0.05). The increases in plasma AOPP and MDA levels as well as renal oxidative stress in DM rats, assessed by DCF-DA staining and urinary 8-OHdG excretion rates, were also significantly attenuated by AST-120 treatment (P < 0.05). CONCLUSION: In conclusion, the renoprotective effects of AST-120 in DM nephropathy seem to be associated with the amelioration of enhanced oxidative stress and FN expression under diabetic conditions.-
dc.description.statementOfResponsibilityopen-
dc.format.extent2134~2141-
dc.relation.isPartOfNEPHROLOGY DIALYSIS TRANSPLANTATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdministration, Oral-
dc.subject.MESHAlbuminuria/metabolism-
dc.subject.MESHAnimals-
dc.subject.MESHCarbon/administration & dosage-
dc.subject.MESHCarbon/pharmacology*-
dc.subject.MESHDeoxyguanosine/analogs & derivatives-
dc.subject.MESHDeoxyguanosine/metabolism-
dc.subject.MESHDiabetic Nephropathies/metabolism*-
dc.subject.MESHDiabetic Nephropathies/physiopathology-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHDisease Progression*-
dc.subject.MESHFibronectins/metabolism*-
dc.subject.MESHMale-
dc.subject.MESHMalondialdehyde/metabolism-
dc.subject.MESHNADPH Oxidases/metabolism-
dc.subject.MESHNitric Oxide Synthase Type III/metabolism-
dc.subject.MESHOxidative Stress/drug effects*-
dc.subject.MESHOxidative Stress/physiology-
dc.subject.MESHOxides/administration & dosage-
dc.subject.MESHOxides/pharmacology*-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHStreptozocin-
dc.titleEffects of an oral adsorbent on oxidative stress and fibronectin expression in experimental diabetic nephropathy-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorSun Ha Lee-
dc.contributor.googleauthorBo Young Nam-
dc.contributor.googleauthorEa Wha Kang-
dc.contributor.googleauthorSeung Hyeok Han-
dc.contributor.googleauthorJin Ji Li-
dc.contributor.googleauthorDo Hee Kim-
dc.contributor.googleauthorSeung Hye Kim-
dc.contributor.googleauthorSeung-Jae Kwak-
dc.contributor.googleauthorJung Tak Park-
dc.contributor.googleauthorTae Ik Chang-
dc.contributor.googleauthorTae-Hyun Yoo-
dc.contributor.googleauthorDae Suk Han-
dc.contributor.googleauthorShin-Wook Kang-
dc.identifier.doi10.1093/ndt/gfq063-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00053-
dc.contributor.localIdA00170-
dc.contributor.localIdA01251-
dc.contributor.localIdA01654-
dc.contributor.localIdA02526-
dc.contributor.localIdA02692-
dc.contributor.localIdA02908-
dc.contributor.localIdA03486-
dc.contributor.localIdA04272-
dc.contributor.localIdA04304-
dc.contributor.localIdA00665-
dc.contributor.localIdA00395-
dc.relation.journalcodeJ02316-
dc.identifier.eissn1460-2385-
dc.identifier.pmid20157172-
dc.subject.keywordAST-120-
dc.subject.keyworddiabetic nephropathy-
dc.subject.keywordfibronectin-
dc.subject.keywordoxidative stress-
dc.contributor.alternativeNameKang, Shin Wook-
dc.contributor.alternativeNameKwak, Seung Jae-
dc.contributor.alternativeNameKim, Do Hee-
dc.contributor.alternativeNameKim, Seung Hye-
dc.contributor.alternativeNameNam, Bo Young-
dc.contributor.alternativeNamePark, Jung Tak-
dc.contributor.alternativeNameYoo, Tae Hyun-
dc.contributor.alternativeNameLi, Jin Ji-
dc.contributor.alternativeNameLee, Sun Ha-
dc.contributor.alternativeNameChang, Tae Ik-
dc.contributor.alternativeNameHan, Dae Suk-
dc.contributor.alternativeNameHan, Seung Hyeok-
dc.contributor.affiliatedAuthorKang, Shin Wook-
dc.contributor.affiliatedAuthorKwak, Seung Jae-
dc.contributor.affiliatedAuthorNam, Bo Young-
dc.contributor.affiliatedAuthorPark, Jung Tak-
dc.contributor.affiliatedAuthorYoo, Tae Hyun-
dc.contributor.affiliatedAuthorLi, Jin Ji-
dc.contributor.affiliatedAuthorLee, Sun Ha-
dc.contributor.affiliatedAuthorChang, Tae Ik-
dc.contributor.affiliatedAuthorHan, Dae Suk-
dc.contributor.affiliatedAuthorHan, Seung Hyeok-
dc.contributor.affiliatedAuthorKim, Seung Hye-
dc.contributor.affiliatedAuthorKim, Do Hee-
dc.citation.volume25-
dc.citation.number7-
dc.citation.startPage2134-
dc.citation.endPage2141-
dc.identifier.bibliographicCitationNEPHROLOGY DIALYSIS TRANSPLANTATION, Vol.25(7) : 2134-2141, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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